TCRβ enhancer activation occurs in some but not all cells with T cell lineage developmental potential

Hillary H. Norris, Lonnie Lybarger, Aaron J. Martin, Hanne Andersen, Deborah C. Chervenak, Robert Chervenak

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Previous studies have shown that murine bone marrow contains a fraction of CD3-/B220-/Thy1lo cells that have pre T cell activity following adoptive transfer and produce sterile transcripts of the T cell receptor β chain gene. The relationship between progenitors and TCRβ transcription has not been examined. Transgenic mice were generated that express green fluorescent protein under the control of the TCRβ enhancer (Eβ). Phenotypic analysis of the founders revealed faithful expression of GFP in populations that express TCRβ transcripts. Examination of the bone marrow showed two populations, CD3-/B220-/Thy1- and CD3-/B220-/Thy1lo, which were GFP+. Both populations were analyzed for their developmental potential following intrathymic transfer into recipient mice. Surprisingly, the GFP+/CD3-/B220-/Thy1lo cells failed to reconstitute; however, the GFP+/CD3-/B220-/Thy1- cells exhibited thymic repopulation. These data demonstrate that Eβ is active pre-thymically; however, pre-thymic transcription of the TCRβ chain gene is neither required for T cell development, nor is it limited to pre T cells.

Original languageEnglish (US)
Pages (from-to)164-174
Number of pages11
JournalCellular Immunology
Volume222
Issue number2
DOIs
StatePublished - Apr 2003
Externally publishedYes

Fingerprint

Cell Lineage
T-Lymphocytes
Population
Bone Marrow Examination
T-Cell Receptor Genes
Adoptive Transfer
Green Fluorescent Proteins
Transgenic Mice
Bone Marrow
Genes

Keywords

  • Hematopoiesis
  • Rodent
  • Stem cells
  • T cell receptors
  • T lymphocytes
  • Transgenic

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

TCRβ enhancer activation occurs in some but not all cells with T cell lineage developmental potential. / Norris, Hillary H.; Lybarger, Lonnie; Martin, Aaron J.; Andersen, Hanne; Chervenak, Deborah C.; Chervenak, Robert.

In: Cellular Immunology, Vol. 222, No. 2, 04.2003, p. 164-174.

Research output: Contribution to journalArticle

Norris, Hillary H. ; Lybarger, Lonnie ; Martin, Aaron J. ; Andersen, Hanne ; Chervenak, Deborah C. ; Chervenak, Robert. / TCRβ enhancer activation occurs in some but not all cells with T cell lineage developmental potential. In: Cellular Immunology. 2003 ; Vol. 222, No. 2. pp. 164-174.
@article{ef0107f7b1e14cf8a069a4e4e3a0d23d,
title = "TCRβ enhancer activation occurs in some but not all cells with T cell lineage developmental potential",
abstract = "Previous studies have shown that murine bone marrow contains a fraction of CD3-/B220-/Thy1lo cells that have pre T cell activity following adoptive transfer and produce sterile transcripts of the T cell receptor β chain gene. The relationship between progenitors and TCRβ transcription has not been examined. Transgenic mice were generated that express green fluorescent protein under the control of the TCRβ enhancer (Eβ). Phenotypic analysis of the founders revealed faithful expression of GFP in populations that express TCRβ transcripts. Examination of the bone marrow showed two populations, CD3-/B220-/Thy1- and CD3-/B220-/Thy1lo, which were GFP+. Both populations were analyzed for their developmental potential following intrathymic transfer into recipient mice. Surprisingly, the GFP+/CD3-/B220-/Thy1lo cells failed to reconstitute; however, the GFP+/CD3-/B220-/Thy1- cells exhibited thymic repopulation. These data demonstrate that Eβ is active pre-thymically; however, pre-thymic transcription of the TCRβ chain gene is neither required for T cell development, nor is it limited to pre T cells.",
keywords = "Hematopoiesis, Rodent, Stem cells, T cell receptors, T lymphocytes, Transgenic",
author = "Norris, {Hillary H.} and Lonnie Lybarger and Martin, {Aaron J.} and Hanne Andersen and Chervenak, {Deborah C.} and Robert Chervenak",
year = "2003",
month = "4",
doi = "10.1016/S0008-8749(03)00113-8",
language = "English (US)",
volume = "222",
pages = "164--174",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - TCRβ enhancer activation occurs in some but not all cells with T cell lineage developmental potential

AU - Norris, Hillary H.

AU - Lybarger, Lonnie

AU - Martin, Aaron J.

AU - Andersen, Hanne

AU - Chervenak, Deborah C.

AU - Chervenak, Robert

PY - 2003/4

Y1 - 2003/4

N2 - Previous studies have shown that murine bone marrow contains a fraction of CD3-/B220-/Thy1lo cells that have pre T cell activity following adoptive transfer and produce sterile transcripts of the T cell receptor β chain gene. The relationship between progenitors and TCRβ transcription has not been examined. Transgenic mice were generated that express green fluorescent protein under the control of the TCRβ enhancer (Eβ). Phenotypic analysis of the founders revealed faithful expression of GFP in populations that express TCRβ transcripts. Examination of the bone marrow showed two populations, CD3-/B220-/Thy1- and CD3-/B220-/Thy1lo, which were GFP+. Both populations were analyzed for their developmental potential following intrathymic transfer into recipient mice. Surprisingly, the GFP+/CD3-/B220-/Thy1lo cells failed to reconstitute; however, the GFP+/CD3-/B220-/Thy1- cells exhibited thymic repopulation. These data demonstrate that Eβ is active pre-thymically; however, pre-thymic transcription of the TCRβ chain gene is neither required for T cell development, nor is it limited to pre T cells.

AB - Previous studies have shown that murine bone marrow contains a fraction of CD3-/B220-/Thy1lo cells that have pre T cell activity following adoptive transfer and produce sterile transcripts of the T cell receptor β chain gene. The relationship between progenitors and TCRβ transcription has not been examined. Transgenic mice were generated that express green fluorescent protein under the control of the TCRβ enhancer (Eβ). Phenotypic analysis of the founders revealed faithful expression of GFP in populations that express TCRβ transcripts. Examination of the bone marrow showed two populations, CD3-/B220-/Thy1- and CD3-/B220-/Thy1lo, which were GFP+. Both populations were analyzed for their developmental potential following intrathymic transfer into recipient mice. Surprisingly, the GFP+/CD3-/B220-/Thy1lo cells failed to reconstitute; however, the GFP+/CD3-/B220-/Thy1- cells exhibited thymic repopulation. These data demonstrate that Eβ is active pre-thymically; however, pre-thymic transcription of the TCRβ chain gene is neither required for T cell development, nor is it limited to pre T cells.

KW - Hematopoiesis

KW - Rodent

KW - Stem cells

KW - T cell receptors

KW - T lymphocytes

KW - Transgenic

UR - http://www.scopus.com/inward/record.url?scp=0037783959&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037783959&partnerID=8YFLogxK

U2 - 10.1016/S0008-8749(03)00113-8

DO - 10.1016/S0008-8749(03)00113-8

M3 - Article

C2 - 12826086

AN - SCOPUS:0037783959

VL - 222

SP - 164

EP - 174

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 2

ER -