Technical note

Method for isolation of the bovine sweat gland and conditions for in vitro culture

S. Hamzaoui, C. A. Burger, J. L. Collier, Robert J Collier

Research output: Contribution to journalArticle

Abstract

Apocrine sweat glands in bovine skin are involved in thermoregulation. Human, horse, and sheep sweat gland epithelial cells have been isolated and grown in vitro. The present study was conducted to identify a method to isolate bovine sweat glands and culture apocrine bovine sweat gland epithelial cells in vitro. Mechanical shearing, collagenase digestion, centrifugation, and neutral red staining were used to identify and isolate the apocrine glands from skin. Bovine sweat glands in situ and after isolation comprised 2 major cell types consisting of a single layer of cuboidal epithelial cells resting on a layer of myoepithelial cells. In situ, the glands were embedded in a collagen matrix primarily comprising fibroblasts, and some of these cells were also present in the isolated material. The isolated material was transferred to complete medium (keratinocyte serum-free medium, bovine pituitary extract, and human recombinant epidermal growth factor + 2.5% fetal bovine serum) in a T 25 flask (Falcon, Franklin Lakes, NJ) with media film and then incubated at 37°C for 24 h. After sweat glands adhered to the bottom of the flask, an additional 2 mL of complete medium was added and the medium was changed every 3 d. Isolated apocrine sweat glands and bovine sweat gland epithelial cells were immunostained for cytokeratin and fibroblast specific protein, indicating fibroblast-free cultures.

Original languageEnglish (US)
Pages (from-to)4638-4642
Number of pages5
JournalJournal of Dairy Science
Volume101
Issue number5
DOIs
StatePublished - May 1 2018

Fingerprint

sweat glands
Sweat Glands
isolation techniques
in vitro culture
cattle
Apocrine Glands
epithelial cells
Epithelial Cells
fibroblasts
Fibroblasts
skin (animal)
Neutral Red
Skin
falcons
collagenase
In Vitro Techniques
Body Temperature Regulation
keratinocytes
Serum-Free Culture Media
epidermal growth factor

Keywords

  • apocrine sweat gland
  • bovine
  • isolation

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Technical note : Method for isolation of the bovine sweat gland and conditions for in vitro culture. / Hamzaoui, S.; Burger, C. A.; Collier, J. L.; Collier, Robert J.

In: Journal of Dairy Science, Vol. 101, No. 5, 01.05.2018, p. 4638-4642.

Research output: Contribution to journalArticle

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