Techniques to measure pilus retraction forces

Nicolas Biais, Dustin Higashi, Magdalene "Maggie" So, Benoit Ladoux

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Citations (Scopus)

Abstract

The importance of physical forces in biology is becoming more appreciated. Neisseria gonorrhoeaehas become a paradigm for the study of physical forces in the bacterial world. Cycles of elongations and retractions of Type IV pili enables N. gonorrhoeaebacteria to exert forces on its environment, forces that play major roles in the life cycle of this pathogen. In order to better understand the role of these forces, there is a need to fully characterize them. Here, we present two different techniques, optical tweezers and Polyacrylamide MicroPillars (PoMPs), for measuring pilus retraction forces. Initially designed for N. gonorrhoeae, these assays can be readily modified to study other pilus-bearing bacteria including Neisseria meningitidis.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages197-216
Number of pages20
Volume799
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume799
ISSN (Print)10643745

Fingerprint

Optical Tweezers
Neisseria
Neisseria meningitidis
Gonorrhea
Life Cycle Stages
Bacteria
polyacrylamide

Keywords

  • Neisseria
  • Optical tweezer
  • PoMPs
  • Retraction force
  • Type IV pilus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Biais, N., Higashi, D., So, M. M., & Ladoux, B. (2012). Techniques to measure pilus retraction forces. In Methods in Molecular Biology (Vol. 799, pp. 197-216). (Methods in Molecular Biology; Vol. 799). https://doi.org/10.1007/978-1-61779-346-2_13

Techniques to measure pilus retraction forces. / Biais, Nicolas; Higashi, Dustin; So, Magdalene "Maggie"; Ladoux, Benoit.

Methods in Molecular Biology. Vol. 799 2012. p. 197-216 (Methods in Molecular Biology; Vol. 799).

Research output: Chapter in Book/Report/Conference proceedingChapter

Biais, N, Higashi, D, So, MM & Ladoux, B 2012, Techniques to measure pilus retraction forces. in Methods in Molecular Biology. vol. 799, Methods in Molecular Biology, vol. 799, pp. 197-216. https://doi.org/10.1007/978-1-61779-346-2_13
Biais N, Higashi D, So MM, Ladoux B. Techniques to measure pilus retraction forces. In Methods in Molecular Biology. Vol. 799. 2012. p. 197-216. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-346-2_13
Biais, Nicolas ; Higashi, Dustin ; So, Magdalene "Maggie" ; Ladoux, Benoit. / Techniques to measure pilus retraction forces. Methods in Molecular Biology. Vol. 799 2012. pp. 197-216 (Methods in Molecular Biology).
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