Temporal assessment of nanoparticle accumulation after experimental brain injury: Effect of particle size

Vimala N. Bharadwaj, Jonathan Lifshitz, P. David Adelson, Vikram D. Kodibagkar, Sarah E. Stabenfeldt

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Nanoparticle (NP) based therapeutic and theranostic agents have been developed for various diseases, yet application to neural disease/injury is restricted by the blood-brain-barrier (BBB). Traumatic brain injury (TBI) results in a host of pathological alterations, including transient breakdown of the BBB, thus opening a window for NP delivery to the injured brain tissue. This study focused on investigating the spatiotemporal accumulation of different sized NPs after TBI. Specifically, animal cohorts sustaining a controlled cortical impact injury received an intravenous injection of PEGylated NP cocktail (20, 40, 100, and 500 nm, each with a unique fluorophore) immediately (0 h), 2 h, 5 h, 12 h, or 23 h after injury. NPs were allowed to circulate for 1 h before perfusion and brain harvest. Confocal microscopy demonstrated peak NP accumulation within the injury penumbra 1 h post-injury. An inverse relationship was found between NP size and their continued accumulation within the penumbra. NP accumulation preferentially occurred in the primary motor and somatosensory areas of the injury penumbra as compared to the parietal association and visual area. Thus, we characterized the accumulation of particles up to 500 nm at different times acutely after injury, indicating the potential of NP-based TBI theranostics in the acute period after injury.

Original languageEnglish (US)
Article number29988
JournalScientific Reports
Volume6
DOIs
StatePublished - Jul 22 2016

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Particle Size
Nanoparticles
Brain Injuries
Wounds and Injuries
Blood-Brain Barrier
Somatosensory Cortex
Motor Cortex
Brain
Confocal Microscopy
Intravenous Injections
Perfusion
Traumatic Brain Injury

ASJC Scopus subject areas

  • General

Cite this

Temporal assessment of nanoparticle accumulation after experimental brain injury : Effect of particle size. / Bharadwaj, Vimala N.; Lifshitz, Jonathan; Adelson, P. David; Kodibagkar, Vikram D.; Stabenfeldt, Sarah E.

In: Scientific Reports, Vol. 6, 29988, 22.07.2016.

Research output: Contribution to journalArticle

Bharadwaj, Vimala N. ; Lifshitz, Jonathan ; Adelson, P. David ; Kodibagkar, Vikram D. ; Stabenfeldt, Sarah E. / Temporal assessment of nanoparticle accumulation after experimental brain injury : Effect of particle size. In: Scientific Reports. 2016 ; Vol. 6.
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