Temporal progression of gene expression responses to salt shock in maize roots

Hong Wang, Saori Miyazaki, Kiyoshi Kawai, Michael Deyholos, David W Galbraith, Hans J. Bohnert

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Using a cDNA microarray containing 7943 ESTs, the behavior of the maize root transcriptome has been monitored in a time course for 72 h after imposition of salinity stress (150 mM NaCl). Under these conditions, root sodium amounts increased faster than in leaves, and root potassium decreased significantly. Although the overall free amino acid concentration was not affected, amino acid composition was changed with proline and asparagine increasing. Microarray analysis identified 916 ESTs representing genes whose steady-state RNA levels were significantly altered at various time points, corresponding to 11% of the ESTs printed. The response of the transcriptome to sub-lethal salt stress was rapid and transient, leading to a burst of changes at the three-hour time point. The salt-regulated ESTs represented 472 tentatively unique genes (TUGs), which, based on functional category analysis, are involved in a broad range of cellular and biochemical activities, prominent amongst which were transport and signal transduction pathways. Clustering of regulated transcripts based on the timing and duration of changes suggests a structured succession of induction and repression for salt responsive genes in multiple signal and response cascades. Within this framework, 16 signaling molecules, including six protein kinases, two protein phosphatases and eight transcription factors, were regulated with distinct expression patterns by high salinity.

Original languageEnglish (US)
Pages (from-to)873-891
Number of pages19
JournalPlant Molecular Biology
Volume52
Issue number4
DOIs
StatePublished - Jul 2003

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Expressed Sequence Tags
Gene expression
Zea mays
Shock
Salts
salts
Gene Expression
transcriptome
gene expression
salt stress
corn
Genes
Salinity
Microarrays
Transcriptome
genes
asparagine
lethal genes
amino acid composition
Amino Acids

Keywords

  • Gene expression profile
  • Microarray
  • Salt stress
  • Time course
  • Zea mays

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Temporal progression of gene expression responses to salt shock in maize roots. / Wang, Hong; Miyazaki, Saori; Kawai, Kiyoshi; Deyholos, Michael; Galbraith, David W; Bohnert, Hans J.

In: Plant Molecular Biology, Vol. 52, No. 4, 07.2003, p. 873-891.

Research output: Contribution to journalArticle

Wang, Hong ; Miyazaki, Saori ; Kawai, Kiyoshi ; Deyholos, Michael ; Galbraith, David W ; Bohnert, Hans J. / Temporal progression of gene expression responses to salt shock in maize roots. In: Plant Molecular Biology. 2003 ; Vol. 52, No. 4. pp. 873-891.
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