Testing the oxidative stress hypothesis of aging in primate fibroblasts: Is there a correlation between species longevity and cellular ROS production?

Anna Csiszar, Andrej Podlutsky, Natalia Podlutskaya, William E. Sonntag, Steven Z. Merlin, Eva E R Philipp, Kristian Doyle, Antonio Davila, Fabio A. Recchia, Praveen Ballabh, John T. Pinto, Zoltan Ungvari

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The present study was conducted to test predictions of the oxidative stress theory of aging assessing reactive oxygen species production and oxidative stress resistance in cultured fibroblasts from 13 primate species ranging in body size from 0.25 to 120 kg and in longevity from 20 to 90 years. We assessed both basal and stress-induced reactive oxygen species production in fibroblasts from five great apes (human, chimpanzee, bonobo, gorilla, and orangutan), four Old World monkeys (baboon, rhesus and crested black macaques, and patas monkey), three New World monkeys (common marmoset, red-bellied tamarin, and woolly monkey), and one lemur (ring-tailed lemur). Measurements of cellular MitoSox fluorescence, an indicator of mitochondrial superoxide (O2 •-) generation, showed an inverse correlation between longevity and steady state or metabolic stress - induced mitochondrial O2 •- production, but this correlation was lost when the effects of body mass were removed, and the data were analyzed using phylogenetically independent contrasts. Fibroblasts from longer-lived primate species also exhibited superior resistance to H2O2-induced apoptotic cell death than cells from shorter-living primates. After correction for body mass and lack of phylogenetic independence, this correlation, although still discernible, fell short of significance by regression analysis. Thus, increased longevity in this sample of primates is not causally associated with low cellular reactive oxygen species generation, but further studies are warranted to test the association between increased cellular resistance to oxidative stressor and primate longevity.

Original languageEnglish (US)
Pages (from-to)841-852
Number of pages12
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume67 A
Issue number8
DOIs
StatePublished - Aug 2012
Externally publishedYes

Fingerprint

Primates
Oxidative Stress
Fibroblasts
Lemur
Reactive Oxygen Species
Erythrocebus patas
Pan paniscus
Atelinae
Pongo
Platyrrhini
Cercopithecidae
Gorilla gorilla
Callithrix
Physiological Stress
Pan troglodytes
Papio
Hominidae
Macaca
Body Size
Superoxides

Keywords

  • Comparative biology
  • Free radical
  • Oxidative stress
  • Primates

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology
  • Medicine(all)

Cite this

Testing the oxidative stress hypothesis of aging in primate fibroblasts : Is there a correlation between species longevity and cellular ROS production? / Csiszar, Anna; Podlutsky, Andrej; Podlutskaya, Natalia; Sonntag, William E.; Merlin, Steven Z.; Philipp, Eva E R; Doyle, Kristian; Davila, Antonio; Recchia, Fabio A.; Ballabh, Praveen; Pinto, John T.; Ungvari, Zoltan.

In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 67 A, No. 8, 08.2012, p. 841-852.

Research output: Contribution to journalArticle

Csiszar, A, Podlutsky, A, Podlutskaya, N, Sonntag, WE, Merlin, SZ, Philipp, EER, Doyle, K, Davila, A, Recchia, FA, Ballabh, P, Pinto, JT & Ungvari, Z 2012, 'Testing the oxidative stress hypothesis of aging in primate fibroblasts: Is there a correlation between species longevity and cellular ROS production?', Journals of Gerontology - Series A Biological Sciences and Medical Sciences, vol. 67 A, no. 8, pp. 841-852. https://doi.org/10.1093/gerona/glr216
Csiszar, Anna ; Podlutsky, Andrej ; Podlutskaya, Natalia ; Sonntag, William E. ; Merlin, Steven Z. ; Philipp, Eva E R ; Doyle, Kristian ; Davila, Antonio ; Recchia, Fabio A. ; Ballabh, Praveen ; Pinto, John T. ; Ungvari, Zoltan. / Testing the oxidative stress hypothesis of aging in primate fibroblasts : Is there a correlation between species longevity and cellular ROS production?. In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences. 2012 ; Vol. 67 A, No. 8. pp. 841-852.
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