Tetraethylammonium transport by snake renal brush-border membrane vesicles

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Transport of tetraethylammonium (TEA) by snake (Thamnophis spp.) renal brush-border membrane vesicles (BBMV) was studied. An outwardly directed proton gradient (pH 6.0 in, pH 7.5 out) stimulated uptake of TEA into BBMV and supported concentrative accumulation. Uptake of radioactively labeled TEA was also stimulated by outwardly directed gradients of unlabeled TEA and choline. The initial rate of TEA uptake was a saturable process that was adequately described by Michaelis-Menten kinetics. TEA uptake was not influenced by changes in the electrical potential difference across the membranes. Although uptake of TEA was stimulated by an outwardly directed Na+ gradient and inhibited by an inwardly directed Na+ gradient, these effects were probably secondary to the generation of proton gradients via a Na+/H+ exchanger demonstrated in these same BBMV. In agreement with previous studies with intact snake renal tubules, the present results indicate that TEA transport across the brush-border membrane involves electroneutral countertransport for protons or organic cations.

Original languageEnglish (US)
Pages (from-to)325-332
Number of pages8
JournalPflugers Archiv European Journal of Physiology
Volume418
Issue number4
DOIs
StatePublished - May 1991

Fingerprint

Tetraethylammonium
Snakes
Brushes
Microvilli
Membranes
Kidney
Protons
Colubridae
Sodium-Hydrogen Antiporter
Proton-Motive Force
Choline
Cations
Kinetics

Keywords

  • Comparative renal physiology
  • Countertransport
  • Garter snake
  • Na/H exchange
  • Organic cation transport
  • Thamnophis spp

ASJC Scopus subject areas

  • Physiology

Cite this

Tetraethylammonium transport by snake renal brush-border membrane vesicles. / Dantzler, William H; Wright, Stephen; Brokl, Olga H.

In: Pflugers Archiv European Journal of Physiology, Vol. 418, No. 4, 05.1991, p. 325-332.

Research output: Contribution to journalArticle

@article{0d3ac303ad3d4ea08f7914818a3634a6,
title = "Tetraethylammonium transport by snake renal brush-border membrane vesicles",
abstract = "Transport of tetraethylammonium (TEA) by snake (Thamnophis spp.) renal brush-border membrane vesicles (BBMV) was studied. An outwardly directed proton gradient (pH 6.0 in, pH 7.5 out) stimulated uptake of TEA into BBMV and supported concentrative accumulation. Uptake of radioactively labeled TEA was also stimulated by outwardly directed gradients of unlabeled TEA and choline. The initial rate of TEA uptake was a saturable process that was adequately described by Michaelis-Menten kinetics. TEA uptake was not influenced by changes in the electrical potential difference across the membranes. Although uptake of TEA was stimulated by an outwardly directed Na+ gradient and inhibited by an inwardly directed Na+ gradient, these effects were probably secondary to the generation of proton gradients via a Na+/H+ exchanger demonstrated in these same BBMV. In agreement with previous studies with intact snake renal tubules, the present results indicate that TEA transport across the brush-border membrane involves electroneutral countertransport for protons or organic cations.",
keywords = "Comparative renal physiology, Countertransport, Garter snake, Na/H exchange, Organic cation transport, Thamnophis spp",
author = "Dantzler, {William H} and Stephen Wright and Brokl, {Olga H.}",
year = "1991",
month = "5",
doi = "10.1007/BF00550869",
language = "English (US)",
volume = "418",
pages = "325--332",
journal = "Pflugers Archiv European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - Tetraethylammonium transport by snake renal brush-border membrane vesicles

AU - Dantzler, William H

AU - Wright, Stephen

AU - Brokl, Olga H.

PY - 1991/5

Y1 - 1991/5

N2 - Transport of tetraethylammonium (TEA) by snake (Thamnophis spp.) renal brush-border membrane vesicles (BBMV) was studied. An outwardly directed proton gradient (pH 6.0 in, pH 7.5 out) stimulated uptake of TEA into BBMV and supported concentrative accumulation. Uptake of radioactively labeled TEA was also stimulated by outwardly directed gradients of unlabeled TEA and choline. The initial rate of TEA uptake was a saturable process that was adequately described by Michaelis-Menten kinetics. TEA uptake was not influenced by changes in the electrical potential difference across the membranes. Although uptake of TEA was stimulated by an outwardly directed Na+ gradient and inhibited by an inwardly directed Na+ gradient, these effects were probably secondary to the generation of proton gradients via a Na+/H+ exchanger demonstrated in these same BBMV. In agreement with previous studies with intact snake renal tubules, the present results indicate that TEA transport across the brush-border membrane involves electroneutral countertransport for protons or organic cations.

AB - Transport of tetraethylammonium (TEA) by snake (Thamnophis spp.) renal brush-border membrane vesicles (BBMV) was studied. An outwardly directed proton gradient (pH 6.0 in, pH 7.5 out) stimulated uptake of TEA into BBMV and supported concentrative accumulation. Uptake of radioactively labeled TEA was also stimulated by outwardly directed gradients of unlabeled TEA and choline. The initial rate of TEA uptake was a saturable process that was adequately described by Michaelis-Menten kinetics. TEA uptake was not influenced by changes in the electrical potential difference across the membranes. Although uptake of TEA was stimulated by an outwardly directed Na+ gradient and inhibited by an inwardly directed Na+ gradient, these effects were probably secondary to the generation of proton gradients via a Na+/H+ exchanger demonstrated in these same BBMV. In agreement with previous studies with intact snake renal tubules, the present results indicate that TEA transport across the brush-border membrane involves electroneutral countertransport for protons or organic cations.

KW - Comparative renal physiology

KW - Countertransport

KW - Garter snake

KW - Na/H exchange

KW - Organic cation transport

KW - Thamnophis spp

UR - http://www.scopus.com/inward/record.url?scp=0025911427&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025911427&partnerID=8YFLogxK

U2 - 10.1007/BF00550869

DO - 10.1007/BF00550869

M3 - Article

VL - 418

SP - 325

EP - 332

JO - Pflugers Archiv European Journal of Physiology

JF - Pflugers Archiv European Journal of Physiology

SN - 0031-6768

IS - 4

ER -