Textural guidance cues for controlling process outgrowth of mammalian neurons

Jennifer N. Hanson, Michael J. Motala, Michael L Heien, Martha Gillette, Jonathan Sweedler, Ralph G. Nuzzo

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

We explore textural cues as a mechanism for controlling neuronal process outgrowth in primary cultures of mammalian neurons. The work uses a form of decal transfer lithography to generate arrays of PDMS posts of various dimensions and spacings on glass substrates that are rendered growth-compliant by subsequent treatment with a protein activator. Hippocampal neurons plated on these substrates are used to determine how the posts direct process growth by acting as attachment points or guidance cues. Textural features varying over a large range, even as large as 100 μm in diameter, dramatically affect process growth. Indeed, two growth regimes are observed; at the smaller feature sizes considered, process branching strongly aligns (at right angles) along the post mesh, while neuronal outgrowth on the larger feature sizes elicits process wrapping. The latter behavior most strongly manifests in neurons plated initially at ∼100 cells/mm2, where the cells were able to form networks, while for isolated neurons, the cells exhibit poorer viability and development. Bag cell neurons from Aplysia californica also display regular growth patterns, but in this case are guided by contact avoidance of the posts, a behavior qualitatively different than that of the hippocampal neurons.

Original languageEnglish (US)
Pages (from-to)122-131
Number of pages10
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume9
Issue number1
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Neurons
Cues
Growth
Aplysia
Substrates
Lithography
Glass
Proteins
Neuronal Outgrowth

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Textural guidance cues for controlling process outgrowth of mammalian neurons. / Hanson, Jennifer N.; Motala, Michael J.; Heien, Michael L; Gillette, Martha; Sweedler, Jonathan; Nuzzo, Ralph G.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 9, No. 1, 2009, p. 122-131.

Research output: Contribution to journalArticle

Hanson, Jennifer N. ; Motala, Michael J. ; Heien, Michael L ; Gillette, Martha ; Sweedler, Jonathan ; Nuzzo, Ralph G. / Textural guidance cues for controlling process outgrowth of mammalian neurons. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2009 ; Vol. 9, No. 1. pp. 122-131.
@article{7c9634a1c63343deb568abefdea8c80d,
title = "Textural guidance cues for controlling process outgrowth of mammalian neurons",
abstract = "We explore textural cues as a mechanism for controlling neuronal process outgrowth in primary cultures of mammalian neurons. The work uses a form of decal transfer lithography to generate arrays of PDMS posts of various dimensions and spacings on glass substrates that are rendered growth-compliant by subsequent treatment with a protein activator. Hippocampal neurons plated on these substrates are used to determine how the posts direct process growth by acting as attachment points or guidance cues. Textural features varying over a large range, even as large as 100 μm in diameter, dramatically affect process growth. Indeed, two growth regimes are observed; at the smaller feature sizes considered, process branching strongly aligns (at right angles) along the post mesh, while neuronal outgrowth on the larger feature sizes elicits process wrapping. The latter behavior most strongly manifests in neurons plated initially at ∼100 cells/mm2, where the cells were able to form networks, while for isolated neurons, the cells exhibit poorer viability and development. Bag cell neurons from Aplysia californica also display regular growth patterns, but in this case are guided by contact avoidance of the posts, a behavior qualitatively different than that of the hippocampal neurons.",
author = "Hanson, {Jennifer N.} and Motala, {Michael J.} and Heien, {Michael L} and Martha Gillette and Jonathan Sweedler and Nuzzo, {Ralph G.}",
year = "2009",
doi = "10.1039/b803595d",
language = "English (US)",
volume = "9",
pages = "122--131",
journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "1",

}

TY - JOUR

T1 - Textural guidance cues for controlling process outgrowth of mammalian neurons

AU - Hanson, Jennifer N.

AU - Motala, Michael J.

AU - Heien, Michael L

AU - Gillette, Martha

AU - Sweedler, Jonathan

AU - Nuzzo, Ralph G.

PY - 2009

Y1 - 2009

N2 - We explore textural cues as a mechanism for controlling neuronal process outgrowth in primary cultures of mammalian neurons. The work uses a form of decal transfer lithography to generate arrays of PDMS posts of various dimensions and spacings on glass substrates that are rendered growth-compliant by subsequent treatment with a protein activator. Hippocampal neurons plated on these substrates are used to determine how the posts direct process growth by acting as attachment points or guidance cues. Textural features varying over a large range, even as large as 100 μm in diameter, dramatically affect process growth. Indeed, two growth regimes are observed; at the smaller feature sizes considered, process branching strongly aligns (at right angles) along the post mesh, while neuronal outgrowth on the larger feature sizes elicits process wrapping. The latter behavior most strongly manifests in neurons plated initially at ∼100 cells/mm2, where the cells were able to form networks, while for isolated neurons, the cells exhibit poorer viability and development. Bag cell neurons from Aplysia californica also display regular growth patterns, but in this case are guided by contact avoidance of the posts, a behavior qualitatively different than that of the hippocampal neurons.

AB - We explore textural cues as a mechanism for controlling neuronal process outgrowth in primary cultures of mammalian neurons. The work uses a form of decal transfer lithography to generate arrays of PDMS posts of various dimensions and spacings on glass substrates that are rendered growth-compliant by subsequent treatment with a protein activator. Hippocampal neurons plated on these substrates are used to determine how the posts direct process growth by acting as attachment points or guidance cues. Textural features varying over a large range, even as large as 100 μm in diameter, dramatically affect process growth. Indeed, two growth regimes are observed; at the smaller feature sizes considered, process branching strongly aligns (at right angles) along the post mesh, while neuronal outgrowth on the larger feature sizes elicits process wrapping. The latter behavior most strongly manifests in neurons plated initially at ∼100 cells/mm2, where the cells were able to form networks, while for isolated neurons, the cells exhibit poorer viability and development. Bag cell neurons from Aplysia californica also display regular growth patterns, but in this case are guided by contact avoidance of the posts, a behavior qualitatively different than that of the hippocampal neurons.

UR - http://www.scopus.com/inward/record.url?scp=57449103546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57449103546&partnerID=8YFLogxK

U2 - 10.1039/b803595d

DO - 10.1039/b803595d

M3 - Article

C2 - 19209344

AN - SCOPUS:57449103546

VL - 9

SP - 122

EP - 131

JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

SN - 1473-0197

IS - 1

ER -