Texture analysis of speckle in optical coherence tomography images of tissue phantoms

Kirk W. Gossage, Cynthia M. Smith, Elizabeth M. Kanter, Lida P. Hariri, Alice L. Stone, Jeffrey J Rodriguez, Stuart K. Williams, Jennifer K Barton

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Optical coherence tomography (OCT) is an imaging modality capable of acquiring cross-sectional images of tissue using back-reflected light. Conventional OCT images have a resolution of 10-15 μm, and are thus best suited for visualizing tissue layers and structures. OCT images of collagen (with and without endothelial cells) have no resolvable features and may appear to simply show an exponential decrease in intensity with depth. However, examination of these images reveals that they display a characteristic repetitive structure due to speckle.The purpose of this study is to evaluate the application of statistical and spectral texture analysis techniques for differentiating living and non-living tissue phantoms containing various sizes and distributions of scatterers based on speckle content in OCT images. Statistically significant differences between texture parameters and excellent classification rates were obtained when comparing various endothelial cell concentrations ranging from 0 cells/ml to 25 million cells/ml. Statistically significant results and excellent classification rates were also obtained using various sizes of microspheres with concentrations ranging from 0 microspheres/ml to 500 million microspheres/ml. This study has shown that texture analysis of OCT images may be capable of differentiating tissue phantoms containing various sizes and distributions of scatterers.

Original languageEnglish (US)
Pages (from-to)1563-1575
Number of pages13
JournalPhysics in Medicine and Biology
Volume51
Issue number6
DOIs
StatePublished - Mar 21 2006

Fingerprint

Optical tomography
Optical Coherence Tomography
Speckle
textures
Textures
tomography
Tissue
Microspheres
Endothelial cells
Endothelial Cells
Collagen
collagens
cells
scattering
Imaging techniques
Light
examination

ASJC Scopus subject areas

  • Biomedical Engineering
  • Physics and Astronomy (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Texture analysis of speckle in optical coherence tomography images of tissue phantoms. / Gossage, Kirk W.; Smith, Cynthia M.; Kanter, Elizabeth M.; Hariri, Lida P.; Stone, Alice L.; Rodriguez, Jeffrey J; Williams, Stuart K.; Barton, Jennifer K.

In: Physics in Medicine and Biology, Vol. 51, No. 6, 21.03.2006, p. 1563-1575.

Research output: Contribution to journalArticle

Gossage, Kirk W. ; Smith, Cynthia M. ; Kanter, Elizabeth M. ; Hariri, Lida P. ; Stone, Alice L. ; Rodriguez, Jeffrey J ; Williams, Stuart K. ; Barton, Jennifer K. / Texture analysis of speckle in optical coherence tomography images of tissue phantoms. In: Physics in Medicine and Biology. 2006 ; Vol. 51, No. 6. pp. 1563-1575.
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