TGF-β in human milk is associated with wheeze in infancy

Wendy H. Oddy, Marilyn Halonen, Fernando Martinez, I. Carla Lohman, Debra A. Stern, Margaret Kurzius-Spencer, Stefano Guerra, Anne L Wright

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Background: Cytokines secreted in human milk might play important roles in newborn health and in the development of infant immune responses. We investigated the relationship of the concentration and dose of cytokines in human milk to infant wheeze at 1 year of age. Objective: Our objective was to test whether the cytokines in milk could account for some of the apparent protective effect of breast-feeding against wheeze in the first year of life. Methods: Data on breast-feeding and infant wheeze were collected prospectively from birth to 1 year from 243 mothers participating in the Infant Immune Study in Tucson, Arizona. Breast milk samples obtained at a mean age of 11 days post-partum were assayed by means of ELISA for concentrations of TGF-β1, IL-10, TNF-α, and the soluble form of CD14. The dose of each cytokine was assessed for a relationship with wheeze in bivariate and logistic regression analyses. Results: Increasing duration of breast-feeding was significantly associated with a decreased prevalence of wheeze (P = .039). There was wide variability in levels of each cytokine in milk, as well as variability between women in the amount of each cytokine produced. There was a significant inverse association between the dose of TGF-β1 received through milk with the percentage of wheeze (P = .017), and the relationship was linear (P = .006). None of the other cytokines showed a linear relationship with wheeze. In multivariate analyses the risk of wheeze was significantly decreased (odds ratio, 0.22; 95% CI 0.05-0.89; P = .034) with increasing TGF-β1 dose (long breastfeeding and medium-high TGF-β1 level compared with short breast-feeding and low TGF-β1 level). Conclusion: This analysis shows that the dose of TGF-β1 received from milk has a significant relationship with infant wheeze, which might account for at least some of the protective effect of breast-feeding against wheeze.

Original languageEnglish (US)
Pages (from-to)723-728
Number of pages6
JournalJournal of Allergy and Clinical Immunology
Volume112
Issue number4
DOIs
StatePublished - Oct 1 2003

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Human Milk
Breast Feeding
Cytokines
Milk
Child Development
Interleukin-10
Multivariate Analysis
Logistic Models
Enzyme-Linked Immunosorbent Assay
Odds Ratio
Regression Analysis
Mothers
Parturition

Keywords

  • Breast-feeding
  • Cytokines
  • Human milk
  • Infancy
  • TGF-β
  • Wheeze

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

TGF-β in human milk is associated with wheeze in infancy. / Oddy, Wendy H.; Halonen, Marilyn; Martinez, Fernando; Lohman, I. Carla; Stern, Debra A.; Kurzius-Spencer, Margaret; Guerra, Stefano; Wright, Anne L.

In: Journal of Allergy and Clinical Immunology, Vol. 112, No. 4, 01.10.2003, p. 723-728.

Research output: Contribution to journalArticle

Oddy, Wendy H. ; Halonen, Marilyn ; Martinez, Fernando ; Lohman, I. Carla ; Stern, Debra A. ; Kurzius-Spencer, Margaret ; Guerra, Stefano ; Wright, Anne L. / TGF-β in human milk is associated with wheeze in infancy. In: Journal of Allergy and Clinical Immunology. 2003 ; Vol. 112, No. 4. pp. 723-728.
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T1 - TGF-β in human milk is associated with wheeze in infancy

AU - Oddy, Wendy H.

AU - Halonen, Marilyn

AU - Martinez, Fernando

AU - Lohman, I. Carla

AU - Stern, Debra A.

AU - Kurzius-Spencer, Margaret

AU - Guerra, Stefano

AU - Wright, Anne L

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AB - Background: Cytokines secreted in human milk might play important roles in newborn health and in the development of infant immune responses. We investigated the relationship of the concentration and dose of cytokines in human milk to infant wheeze at 1 year of age. Objective: Our objective was to test whether the cytokines in milk could account for some of the apparent protective effect of breast-feeding against wheeze in the first year of life. Methods: Data on breast-feeding and infant wheeze were collected prospectively from birth to 1 year from 243 mothers participating in the Infant Immune Study in Tucson, Arizona. Breast milk samples obtained at a mean age of 11 days post-partum were assayed by means of ELISA for concentrations of TGF-β1, IL-10, TNF-α, and the soluble form of CD14. The dose of each cytokine was assessed for a relationship with wheeze in bivariate and logistic regression analyses. Results: Increasing duration of breast-feeding was significantly associated with a decreased prevalence of wheeze (P = .039). There was wide variability in levels of each cytokine in milk, as well as variability between women in the amount of each cytokine produced. There was a significant inverse association between the dose of TGF-β1 received through milk with the percentage of wheeze (P = .017), and the relationship was linear (P = .006). None of the other cytokines showed a linear relationship with wheeze. In multivariate analyses the risk of wheeze was significantly decreased (odds ratio, 0.22; 95% CI 0.05-0.89; P = .034) with increasing TGF-β1 dose (long breastfeeding and medium-high TGF-β1 level compared with short breast-feeding and low TGF-β1 level). Conclusion: This analysis shows that the dose of TGF-β1 received from milk has a significant relationship with infant wheeze, which might account for at least some of the protective effect of breast-feeding against wheeze.

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