TGF-β1 mediates the hypertrophic cardiomyocyte growth induced by angiotensin II

Jo El J. Schultz, Sandra A. Witt, Betty J. Glascock, Michelle L. Nieman, Peter J. Reiser, Stacey L. Nix, Thomas R. Kimballand, Thomas Doetschman

Research output: Contribution to journalArticlepeer-review

354 Scopus citations

Abstract

Angiotensin II (Ang II), a potent hypertrophic stimulus, causes significant increases in TGFb1 gene expression. However, it is not known whether there is a causal relationship between increased levels of TGF-β1 and cardiac hypertrophy. Echocardiographic analysis revealed that TGF-β-deficient mice subjected to chronic subpressor doses of Ang II had no significant change in left ventricular (LV) mass and percent fractional shortening during Ang II treatment. In contrast, Ang II-treated wild-type mice showed a >20% increase in LV mass and impaired cardiac function. Cardiomyocyte cross-sectional area was also markedly increased in Ang II-treated wild-type mice but unchanged in Ang II-treated TGF-β1-deficient mice. No significant levels of fibrosis, mitotic growth, or cytokine infiltration were detected in Ang II-treated mice. A trial natriuretic factor expression was -6-fold elevated in Ang II-treated wild-type, but not TGF-β1-deficient mice. However, the α- to β-myosin heavy chain switch did not occur in Ang II-treated mice, indicating that isoform switching is not obligatorily coupled with hypertrophy or TGF-β1. The Ang II effect on hypertrophy was shown not to result from stimulation of the endogenous renin-angiotensis system. These results indicate that TGF-β1 is an important mediator of the hypertrophic growth response of the heart to Ang II.

Original languageEnglish (US)
Pages (from-to)787-796
Number of pages10
JournalJournal of Clinical Investigation
Volume109
Issue number6
DOIs
StatePublished - 2002
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'TGF-β1 mediates the hypertrophic cardiomyocyte growth induced by angiotensin II'. Together they form a unique fingerprint.

Cite this