Th1/Th2 patterns and balance in cytokine production in the parents and infants of a large birth cohort

Marilyn Halonen, I. Carla Lohman, Debra A. Stern, Amber Spangenberg, Dayna Anderson, Sara Mobley, Kathy Ciano, Michael Peck, Anne L. Wright

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68 Scopus citations

Abstract

Regulation of human immune cell cytokine production in vivo is not well understood due in part to limitations on imposing experimental conditions. We proposed that life-imposed conditions (pregnancy, birth, age, gender), combined with large sample size, repeat sampling, and family-based recruitment would serve to reveal peripheral blood cell-derived cytokine patterns reflective of in vivo regulation regarding Th1/Th2 balance and familial correlation. Mononuclear cells were obtained from 483 trios in the Tucson Infant Immune Study: from mothers pre- and postpartum, infants at birth and at 3 mo, and fathers. Con A/PMAstimulated supernatants were assayed by ELISA for IFN-γ, IL-4, IL-13, IL-5, and IL-10 and allergen-stimulated supernatants for IFN-γ, IL-4, and IL-13. Mitogen-stimulated prepartum samples were not globally Th2 biased, differing from postpartum only by a modestly reduced IFN-γ:IL-5 ratio. Prepartum samples actually produced less IL-10 and IL-13 although more IL-5 than paternal samples. Newborns were also not globally Th2 biased, with mitogen stimulation producing ∼10-fold less IL-4, IL-5, and IFN-γ than adults but only 2- to 3-fold less IL-13 and IL-10. Despite these group differences, all cytokines showed marked positive intraindividual correlations (all p < 0.001). Allergen stimulation gave results consistent with a lack of global Th2 bias. Mitogen stimulation revealed parent-child and parent-parent correlations. Thus, rather than a global Th2 bias, cytokine production in pregnant mothers and newborns appears regulated so as to maintain a relative balance among the cytokines, with the nature of the balance differing in mothers and infants and with production influenced by familial factors that include shared environment.

Original languageEnglish (US)
Pages (from-to)3285-3293
Number of pages9
JournalJournal of Immunology
Volume182
Issue number5
DOIs
StatePublished - Mar 1 2009

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Halonen, M., Lohman, I. C., Stern, D. A., Spangenberg, A., Anderson, D., Mobley, S., Ciano, K., Peck, M., & Wright, A. L. (2009). Th1/Th2 patterns and balance in cytokine production in the parents and infants of a large birth cohort. Journal of Immunology, 182(5), 3285-3293. https://doi.org/10.4049/jimmunol.0711996