The actin cytoskeleton mediates the hormonally regulated translocation of type II iodothyronine 5′-deiodinase in astrocytes

Alan P. Farwell, Ron Lynch, William C. Okulicz, Ann M. Comi, Jack L. Leonard

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Thyroid hormone, specifically thyroxine, alters cytoskeletal organization in astrocytes by modulating actin polymerization and, in turn, regulates the turnover of the short-lived membrane protein, type II iodothyronine 5′-deiodinase. In the absence of thyroxine, ∼35% of the total cellular actin is depolymerized, and >90% of the deiodinase is found in the plasma membrane and not associated with the cytoskeleton. Addition of thyroxine promotes actin polymerization and decreases the depolymerized actin to ∼10% of the total actin pool, induces binding of the deiodinase to F-actin, and promotes rapid internalization of the enzyme. These data provide direct evidence that the actin cytoskeleton participates in the inactivation pathway of the deiodinase by translocating this short-lived plasma membrane protein to an internal membrane pool.

Original languageEnglish (US)
Pages (from-to)18546-18553
Number of pages8
JournalJournal of Biological Chemistry
Volume265
Issue number30
StatePublished - Oct 25 1990
Externally publishedYes

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Iodide Peroxidase
Actin Cytoskeleton
Astrocytes
Actins
Thyroxine
Polymerization
Cell membranes
Membrane Proteins
Cell Membrane
Cytoskeleton
Thyroid Hormones
iodothyronine deiodinase type II
Blood Proteins
Membranes
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

The actin cytoskeleton mediates the hormonally regulated translocation of type II iodothyronine 5′-deiodinase in astrocytes. / Farwell, Alan P.; Lynch, Ron; Okulicz, William C.; Comi, Ann M.; Leonard, Jack L.

In: Journal of Biological Chemistry, Vol. 265, No. 30, 25.10.1990, p. 18546-18553.

Research output: Contribution to journalArticle

Farwell, Alan P. ; Lynch, Ron ; Okulicz, William C. ; Comi, Ann M. ; Leonard, Jack L. / The actin cytoskeleton mediates the hormonally regulated translocation of type II iodothyronine 5′-deiodinase in astrocytes. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 30. pp. 18546-18553.
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