Abstract
CSF-1 mRNA 3′UTR variants (var) are generated from alternative splicing. CSF-1 protein encoded by var-1 mRNA with long 3′UTR derived from exon-10 is rapidly secreted compared to the CSF-1 protein encoded by var-4 mRNA with short 3′UTR derived from exon-9. Secretion kinetics indicates that HuR, which binds the CSF-1 var-1 mRNA, but not var-4 mRNA, accelerates the secretion of CSF-1 protein. HuR overexpression increases the secretion rate of CSF-1 protein. In contrast, silencing of HuR does not have such an effect, suggesting other compensatory mechanisms. Effect of the CSF-1 mRNA variant 3′UTRs on cellular phenotype shows both CSF-1 var-1 or -4 mRNA is involved in the enhanced rates of migration and invasion observed by both in vitro in breast cancer cells. Our study indicates that the alternative splicing of CSF-1 mRNA 3′UTR can regulate differential secretion of CSF-1 protein.
Original language | English (US) |
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Pages (from-to) | 1004-1010 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 525 |
Issue number | 4 |
DOIs | |
State | Published - May 14 2020 |
Keywords
- CSF-1 differential secretion
- HuR
- Var-1 long 3′UTR
- Var-4 short 3′UTR
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology