The AngR protein and the siderophore anguibactin positively regulate the expression of iron-transport genes in Vibrio anguillarum

Qian Chen, Anne M. Wertheimer, Marcelo E. Tolmasky, Jorge H. Crosa

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Vibrio anguillarum virulence is associated with the presence of a plasmid-mediated iron-uptake system expressed under iron-limiting conditions, which consists of the siderophore anguibactin and specific iron-transport proteins. This system is maximally expressed under iron-limiting conditions and requires the AngR protein that acts as a positive regulator of anguibactin biosynthesis and also possess an EntE-like enzymatic function that may play a role in anguibactin biosynthesis. In this work, we demonstrate that in addition to possessing these functions related to anguibactin production, AngR also positively regulated transcription of the iron-transport genes fatA and fatB. We also show that transcription of angR is repressed by Fur under iron-rich conditions. In addition, we present evidence that anguibactin itself enhanced transcription of the iron-transport genes fatA and fatB, independently of AngR and the trans-acting factor (TAF) product(s). The presence of either AngR (together with the TAF product(s)) or anguibactin alone led to a partial level of expression of the iron-transport genes fatA and fatB, while full expression is achieved when AngR, the TAF products and anguibactin are all present.

Original languageEnglish (US)
Pages (from-to)127-134
Number of pages8
JournalMolecular Microbiology
Volume22
Issue number1
DOIs
StatePublished - Oct 21 1996
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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