The anti-mitogenic activity of 17β -estradiol in coronary smooth muscle cells correlates with protein binding to its responsive element

Kenneth Ramos, D. N. Sadhu, W. M. Chilian

Research output: Contribution to journalArticle

Abstract

The cellular and molecular mechanisms responsible for the protection afforded by 17β -estradiol against coronary atherosclerosis in premenopausal women remain undefined. The present studies were conducted to assess the impact of 17β -estradiol (1-100 nM) on coronary smooth muscle cell (c-SMC) proliferation and the integrity of estrogen signaling in these cells. c-SMCs were isolated from 9-month old Sinclair pigs and established in culture by standard procedures. Basal DNA synthetic rates were increased 3-fold by 24 hr in cultures challenged with 1% serum. 17β -estradiol did not modulate basal proliferation rates, but markedly reduced the mitogenic response elicited by serum in a concentration-dependent manner. The anti-mitogenic effects of 17β -estradiol in c-SMCs was compromised when cells were maximally stimulated with serum. Electrophoretic mobility shift analysis of nuclear extracts of c-SMCs using a double-stranded oligonucleotide derived from the estrogen responsive element of the vitellogenin A2 gene confirmed the occurrence of ligand-induced specific protein/DNA complexes. These data implicate anti-mitogenic effects in the protection against coronary atherosclerosis by 17β -estradiol and suggest that the response involves orotein/DNA interactions influenced bv the degree of mitoeenic stimulation of c-SMCs.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

protein binding
Protein Binding
smooth muscle
myocytes
Smooth Muscle Myocytes
Muscle
estradiol
Estradiol
Cells
estrogens
Coronary Artery Disease
DNA
Estrogens
Serum
Vitellogenins
Electrophoretic mobility
vitellogenin
Cell proliferation
oligonucleotides
varespladib methyl

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

The anti-mitogenic activity of 17β -estradiol in coronary smooth muscle cells correlates with protein binding to its responsive element. / Ramos, Kenneth; Sadhu, D. N.; Chilian, W. M.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

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