The application of a poliovirus cDNA probe for the detection of enteroviruses in water

A. B. Margolin, M. J. Hewlett, Charles P Gerba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

A technique for the detection of enteroviruses in water using gene probes in a dot blot assay is described. Poliovirus cDNA probes were labeled with [32P] dCTP and [32P] dATP to a specific activity greater than 2.0 x 109 cmp/μg DNA. Autoradiograph times were compared to determine the minimum time required to optimize the sensitivity of the test. One infectious unit of poliovirus was detected from cell harvests within 38 hours using gene probes. Potential false positive reactions between bacterial DNA in environmental samples and vector DNA were controlled by both base hydrolysis of viral RNA and enzymatic treatment of the sample. Upon comparison to cell culture, the dot blot assay was as sensitive for the detection of virus in sewage contaminated groundwater.

Original languageEnglish (US)
Title of host publicationWater Science and Technology
Pages277-280
Number of pages4
Volume24
Edition2
StatePublished - 1991
Externally publishedYes
EventProceedings of the IAWPRC International Symposium - Tuebingen, Ger
Duration: Apr 1 1990Apr 6 1990

Other

OtherProceedings of the IAWPRC International Symposium
CityTuebingen, Ger
Period4/1/904/6/90

Fingerprint

probe
DNA
assay
gene
water
RNA
hydrolysis
virus
sewage
groundwater
detection
enterovirus
comparison
harvest
test

ASJC Scopus subject areas

  • Water Science and Technology

Cite this

Margolin, A. B., Hewlett, M. J., & Gerba, C. P. (1991). The application of a poliovirus cDNA probe for the detection of enteroviruses in water. In Water Science and Technology (2 ed., Vol. 24, pp. 277-280)

The application of a poliovirus cDNA probe for the detection of enteroviruses in water. / Margolin, A. B.; Hewlett, M. J.; Gerba, Charles P.

Water Science and Technology. Vol. 24 2. ed. 1991. p. 277-280.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Margolin, AB, Hewlett, MJ & Gerba, CP 1991, The application of a poliovirus cDNA probe for the detection of enteroviruses in water. in Water Science and Technology. 2 edn, vol. 24, pp. 277-280, Proceedings of the IAWPRC International Symposium, Tuebingen, Ger, 4/1/90.
Margolin AB, Hewlett MJ, Gerba CP. The application of a poliovirus cDNA probe for the detection of enteroviruses in water. In Water Science and Technology. 2 ed. Vol. 24. 1991. p. 277-280
Margolin, A. B. ; Hewlett, M. J. ; Gerba, Charles P. / The application of a poliovirus cDNA probe for the detection of enteroviruses in water. Water Science and Technology. Vol. 24 2. ed. 1991. pp. 277-280
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