The application of a poliovirus cDNA probe for the detection of enteroviruses in water

A. B. Margolin, M. J. Hewlett, C. P. Gerba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

A technique for the detection of enteroviruses in water using gene probes in a dot blot assay is described. Poliovirus cDNA probes were labeled with [32P] dCTP and [32P] dATP to a specific activity greater than 2.0 × 109 cpm/μg DNA. Autoradiograph times were compared to determine the minimum time required to optimize the sensitivity of the test. One infectious unit of poliovirus was detected from cell harvests within 48 hours using gene probes. Potential false positive reactions between bacterial DNA in environmental samples and vector DNA were controlled by both base hydrolysis of viral RNA and enzymatic treatment of the sample. Upon comparison to cell culture, the dot blot assay was as sensitive for the detection of virus in sewage contaminated groundwater.

Original languageEnglish (US)
Title of host publicationHealth-Related Water Microbiolgy 1990
Pages277-280
Number of pages4
Volume24
Edition2
DOIs
StatePublished - 1991
Externally publishedYes
EventProceedings of the IAWPRC International Symposium - Tuebingen, Ger
Duration: Apr 1 1990Apr 6 1990

Other

OtherProceedings of the IAWPRC International Symposium
CityTuebingen, Ger
Period4/1/904/6/90

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

Fingerprint Dive into the research topics of 'The application of a poliovirus cDNA probe for the detection of enteroviruses in water'. Together they form a unique fingerprint.

  • Cite this

    Margolin, A. B., Hewlett, M. J., & Gerba, C. P. (1991). The application of a poliovirus cDNA probe for the detection of enteroviruses in water. In Health-Related Water Microbiolgy 1990 (2 ed., Vol. 24, pp. 277-280) https://doi.org/10.2166/wst.1991.0073