The application of multiple reaction monitoring to assess ApoA-I methionine oxidations in diabetes and cardiovascular disease

Hussein N. Yassine, Angela M. Jackson, Peter D. Reaven, Dobrin Nedelkov, Randall W. Nelson, Serrine Lau, Christoph H. Borchers

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The oxidative modification of apolipoprotein A-I's methionine148 (M148) is associated with defective HDL function in vitro. Multiple reaction monitoring (MRM) is a mass spectrometric technique that can be used to quantitate post-translational modifications. In this study, we developed an MRM assay to monitor the abundance ratio of the peptide containing oxidized M148 to the native peptide in ApoA-I. Measurement of the oxidized-to-unoxidized-M148 ratio was reproducible (CV < 5%). The extent of methionine M148 oxidation in the HDL of healthy controls, and type 2 diabetic participants with and without prior cardiovascular events (CVD) were then examined. The results suggest a significant increase in the relative ratio of the peptide containing oxidized M148 to the unmodified peptide in the HDL of participants with diabetes and CVD (p < 0.001), compared to participants without CVD. Monitoring the abundance ratio of the peptides containing oxidized and unoxidized M148 by MRM provides a means of examining the relationship between M148 oxidation and vascular complications in CVD.

Original languageEnglish (US)
Pages (from-to)18-24
Number of pages7
JournalTranslational Proteomics
Volume4-5
DOIs
StatePublished - 2014

Fingerprint

Apolipoprotein A-I
Medical problems
Methionine
Cardiovascular Diseases
Chemical vapor deposition
Oxidation
Peptides
Monitoring
Apolipoproteins A
Post Translational Protein Processing
Blood Vessels
Assays

Keywords

  • Cardiovascular disease
  • Diabetes
  • HDL
  • Multiple reaction monitoring
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry

Cite this

The application of multiple reaction monitoring to assess ApoA-I methionine oxidations in diabetes and cardiovascular disease. / Yassine, Hussein N.; Jackson, Angela M.; Reaven, Peter D.; Nedelkov, Dobrin; Nelson, Randall W.; Lau, Serrine; Borchers, Christoph H.

In: Translational Proteomics, Vol. 4-5, 2014, p. 18-24.

Research output: Contribution to journalArticle

Yassine, Hussein N. ; Jackson, Angela M. ; Reaven, Peter D. ; Nedelkov, Dobrin ; Nelson, Randall W. ; Lau, Serrine ; Borchers, Christoph H. / The application of multiple reaction monitoring to assess ApoA-I methionine oxidations in diabetes and cardiovascular disease. In: Translational Proteomics. 2014 ; Vol. 4-5. pp. 18-24.
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AU - Nelson, Randall W.

AU - Lau, Serrine

AU - Borchers, Christoph H.

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