The Arabidopsis chaperone J3 regulates the plasma membrane H+-ATPase through interaction with the PKS5 kinase

Yongqing Yang, Yunxia Qin, Changgen Xie, Feiyi Zhao, Jinfeng Zhao, Dafa Liu, Shouyi Chen, Anja T. Fuglsang, Michael G. Palmgren, Karen S. Schumaker, Xing Wang Deng, Yan Guo

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104 Scopus citations

Abstract

The plasma membrane H+-ATPase (PM H+-ATPase) plays an important role in the regulation of ion and metabolite transport and is involved in physiological processes that include cell growth, intracellular pH, and stomatal regulation. PM H+-ATPase activity is controlled by many factors, including hormones, calcium, light, and environmental stresses like increased soil salinity. We have previously shown that the Arabidopsis thaliana Salt Overly Sensitive2-Like Protein Kinase5 (PKS5) negatively regulates the PM H+-ATPase. Here, we report that a chaperone, J3 (DnaJ homolog 3; heat shock protein 40-like), activates PM H+-ATPase activity by physically interacting with and repressing PKS5 kinase activity. Plants lacking J3 are hypersensitive to salt at high external pH and exhibit decreased PM H+-ATPase activity. J3 functions upstream of PKS5 as double mutants generated using j3-1 and several pks5 mutant alleles with altered kinase activity have levels of PM H+-ATPase activity and responses to salt at alkaline pH similar to their corresponding pks5 mutant. Taken together, our results demonstrate that regulation of PM H+-ATPase activity by J3 takes place via inactivation of the PKS5 kinase.

Original languageEnglish (US)
Pages (from-to)1313-1332
Number of pages20
JournalPlant Cell
Volume22
Issue number4
DOIs
StatePublished - Apr 1 2010

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ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Yang, Y., Qin, Y., Xie, C., Zhao, F., Zhao, J., Liu, D., Chen, S., Fuglsang, A. T., Palmgren, M. G., Schumaker, K. S., Deng, X. W., & Guo, Y. (2010). The Arabidopsis chaperone J3 regulates the plasma membrane H+-ATPase through interaction with the PKS5 kinase. Plant Cell, 22(4), 1313-1332. https://doi.org/10.1105/tpc.109.069609