The Arabidopsis chaperone J3 regulates the plasma membrane H+-ATPase through interaction with the PKS5 kinase

Yongqing Yang, Yunxia Qin, Changgen Xie, Feiyi Zhao, Jinfeng Zhao, Dafa Liu, Shouyi Chen, Anja T. Fuglsang, Michael G. Palmgren, Karen S Schumaker, Xing Wang Deng, Yan Guo

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

The plasma membrane H+-ATPase (PM H+-ATPase) plays an important role in the regulation of ion and metabolite transport and is involved in physiological processes that include cell growth, intracellular pH, and stomatal regulation. PM H+-ATPase activity is controlled by many factors, including hormones, calcium, light, and environmental stresses like increased soil salinity. We have previously shown that the Arabidopsis thaliana Salt Overly Sensitive2-Like Protein Kinase5 (PKS5) negatively regulates the PM H+-ATPase. Here, we report that a chaperone, J3 (DnaJ homolog 3; heat shock protein 40-like), activates PM H+-ATPase activity by physically interacting with and repressing PKS5 kinase activity. Plants lacking J3 are hypersensitive to salt at high external pH and exhibit decreased PM H+-ATPase activity. J3 functions upstream of PKS5 as double mutants generated using j3-1 and several pks5 mutant alleles with altered kinase activity have levels of PM H+-ATPase activity and responses to salt at alkaline pH similar to their corresponding pks5 mutant. Taken together, our results demonstrate that regulation of PM H+-ATPase activity by J3 takes place via inactivation of the PKS5 kinase.

Original languageEnglish (US)
Pages (from-to)1313-1332
Number of pages20
JournalPlant Cell
Volume22
Issue number4
DOIs
StatePublished - Apr 2010

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H-transporting ATP synthase
Proton-Translocating ATPases
Arabidopsis
protein kinases
Protein Kinases
plasma membrane
Cell Membrane
Salts
salts
mutants
Cell Growth Processes
HSP40 Heat-Shock Proteins
Cell Physiological Phenomena
Ion Transport
Salinity
soil salinity
heat shock proteins
cell growth
inactivation
phosphotransferases (kinases)

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

The Arabidopsis chaperone J3 regulates the plasma membrane H+-ATPase through interaction with the PKS5 kinase. / Yang, Yongqing; Qin, Yunxia; Xie, Changgen; Zhao, Feiyi; Zhao, Jinfeng; Liu, Dafa; Chen, Shouyi; Fuglsang, Anja T.; Palmgren, Michael G.; Schumaker, Karen S; Deng, Xing Wang; Guo, Yan.

In: Plant Cell, Vol. 22, No. 4, 04.2010, p. 1313-1332.

Research output: Contribution to journalArticle

Yang, Y, Qin, Y, Xie, C, Zhao, F, Zhao, J, Liu, D, Chen, S, Fuglsang, AT, Palmgren, MG, Schumaker, KS, Deng, XW & Guo, Y 2010, 'The Arabidopsis chaperone J3 regulates the plasma membrane H+-ATPase through interaction with the PKS5 kinase', Plant Cell, vol. 22, no. 4, pp. 1313-1332. https://doi.org/10.1105/tpc.109.069609
Yang, Yongqing ; Qin, Yunxia ; Xie, Changgen ; Zhao, Feiyi ; Zhao, Jinfeng ; Liu, Dafa ; Chen, Shouyi ; Fuglsang, Anja T. ; Palmgren, Michael G. ; Schumaker, Karen S ; Deng, Xing Wang ; Guo, Yan. / The Arabidopsis chaperone J3 regulates the plasma membrane H+-ATPase through interaction with the PKS5 kinase. In: Plant Cell. 2010 ; Vol. 22, No. 4. pp. 1313-1332.
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