The Argonaute-binding platform of NRPE1 evolves through modulation of intrinsically disordered repeats

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Argonaute (Ago) proteins are important effectors in RNA silencing pathways, but they must interact with other machinery to trigger silencing. Ago hooks have emerged as a conserved motif responsible for interaction with Ago proteins, but little is known about the sequence surrounding Ago hooks that must restrict or enable interaction with specific Argonautes. Here we investigated the evolutionary dynamics of an Ago-binding platform in NRPE1, the largest subunit of RNA polymerase V. We compared NRPE1 sequences from > 50 species, including dense sampling of two plant lineages. This study demonstrates that the Ago-binding platform of NRPE1 retains Ago hooks, intrinsic disorder, and repetitive character while being highly labile at the sequence level. We reveal that loss of sequence conservation is the result of relaxed selection and frequent expansions and contractions of tandem repeat arrays. These factors allow a complete restructuring of the Ago-binding platform over 50–60 million yr. This evolutionary pattern is also detected in a second Ago-binding platform, suggesting it is a general mechanism. The presence of labile repeat arrays in all analyzed NRPE1 Ago-binding platforms indicates that selection maintains repetitive character, potentially to retain the ability to rapidly restructure the Ago-binding platform.

Original languageEnglish (US)
Pages (from-to)1094-1105
Number of pages12
JournalNew Phytologist
Volume212
Issue number4
DOIs
StatePublished - Dec 1 2016

Fingerprint

Argonaute Proteins
Tandem Repeat Sequences
tandem repeat sequences
DNA-Directed RNA Polymerases
DNA-directed RNA polymerase
RNA Interference
RNA interference
proteins
sampling

Keywords

  • Ago hook
  • Argonaute
  • intrinsic disorder
  • polymerase V (Pol V)
  • relaxed selection
  • repeat expansion
  • RNA-directed DNA methylation
  • tandem repeat

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

The Argonaute-binding platform of NRPE1 evolves through modulation of intrinsically disordered repeats. / Trujillo, Joshua T.; Beilstein, Mark A; Harris, Rebecca Ann Mosher.

In: New Phytologist, Vol. 212, No. 4, 01.12.2016, p. 1094-1105.

Research output: Contribution to journalArticle

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