The bacterial enhancer-binding protein NTRC is a molecular machine: ATP hydrolysis is coupled to transcriptional activation

Andrew B Wedel, Sydney Kustu

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

NTRC is a prokaryotic enhancer-binding protein that activates transcription by σ54-holoenzyme. NTRC has an ATPase activity that is required for transcriptional activation, specifically for isomerization of closed complexes between σ54-holoenzyme and a promoter to open complexes. In the absence of ATP hydrolysis, there is known to be a kinetic barrier to open complex formation (i.e., the reaction proceeds so slowly that the polymerase synthesizes essentially no transcripts even from a supercoiled template). We show here that open complex formation is also thermodynamically unfavorable. In the absence of ATP hydrolysis the position of equilibrium between closed and open complexes favors the closed ones. Use of linear templates with a region of heteroduplex around the transcriptional start site-'preopened' templates-does not bypass the requirement for either NTRC or ATP hydrolysis, providing evidence that the rate-limiting step in open complex formation does not lie in DNA strand denaturation per se. These results are in contrast to recent findings regarding the ATP requirement for initiation of transcription by eukaryotic RNA polymerase II; in the latter case, the ATP requirement is circumvented by use of a supercoiled plasmid template or a preopened linear template.

Original languageEnglish (US)
Pages (from-to)2042-2052
Number of pages11
JournalGenes and Development
Volume9
Issue number16
DOIs
StatePublished - Aug 15 1995
Externally publishedYes

Fingerprint

Transcriptional Activation
Carrier Proteins
Hydrolysis
Adenosine Triphosphate
Holoenzymes
Nucleic Acid Denaturation
RNA Polymerase II
Adenosine Triphosphatases
Plasmids

Keywords

  • ATPase
  • enhancers
  • enteric bacteria
  • heteroduplex template
  • open complexes
  • σ

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

The bacterial enhancer-binding protein NTRC is a molecular machine : ATP hydrolysis is coupled to transcriptional activation. / Wedel, Andrew B; Kustu, Sydney.

In: Genes and Development, Vol. 9, No. 16, 15.08.1995, p. 2042-2052.

Research output: Contribution to journalArticle

@article{c08532e00d604145bb848a7f844bae8c,
title = "The bacterial enhancer-binding protein NTRC is a molecular machine: ATP hydrolysis is coupled to transcriptional activation",
abstract = "NTRC is a prokaryotic enhancer-binding protein that activates transcription by σ54-holoenzyme. NTRC has an ATPase activity that is required for transcriptional activation, specifically for isomerization of closed complexes between σ54-holoenzyme and a promoter to open complexes. In the absence of ATP hydrolysis, there is known to be a kinetic barrier to open complex formation (i.e., the reaction proceeds so slowly that the polymerase synthesizes essentially no transcripts even from a supercoiled template). We show here that open complex formation is also thermodynamically unfavorable. In the absence of ATP hydrolysis the position of equilibrium between closed and open complexes favors the closed ones. Use of linear templates with a region of heteroduplex around the transcriptional start site-'preopened' templates-does not bypass the requirement for either NTRC or ATP hydrolysis, providing evidence that the rate-limiting step in open complex formation does not lie in DNA strand denaturation per se. These results are in contrast to recent findings regarding the ATP requirement for initiation of transcription by eukaryotic RNA polymerase II; in the latter case, the ATP requirement is circumvented by use of a supercoiled plasmid template or a preopened linear template.",
keywords = "ATPase, enhancers, enteric bacteria, heteroduplex template, open complexes, σ",
author = "Wedel, {Andrew B} and Sydney Kustu",
year = "1995",
month = "8",
day = "15",
doi = "10.1101/gad.9.16.2042",
language = "English (US)",
volume = "9",
pages = "2042--2052",
journal = "Genes and Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "16",

}

TY - JOUR

T1 - The bacterial enhancer-binding protein NTRC is a molecular machine

T2 - ATP hydrolysis is coupled to transcriptional activation

AU - Wedel, Andrew B

AU - Kustu, Sydney

PY - 1995/8/15

Y1 - 1995/8/15

N2 - NTRC is a prokaryotic enhancer-binding protein that activates transcription by σ54-holoenzyme. NTRC has an ATPase activity that is required for transcriptional activation, specifically for isomerization of closed complexes between σ54-holoenzyme and a promoter to open complexes. In the absence of ATP hydrolysis, there is known to be a kinetic barrier to open complex formation (i.e., the reaction proceeds so slowly that the polymerase synthesizes essentially no transcripts even from a supercoiled template). We show here that open complex formation is also thermodynamically unfavorable. In the absence of ATP hydrolysis the position of equilibrium between closed and open complexes favors the closed ones. Use of linear templates with a region of heteroduplex around the transcriptional start site-'preopened' templates-does not bypass the requirement for either NTRC or ATP hydrolysis, providing evidence that the rate-limiting step in open complex formation does not lie in DNA strand denaturation per se. These results are in contrast to recent findings regarding the ATP requirement for initiation of transcription by eukaryotic RNA polymerase II; in the latter case, the ATP requirement is circumvented by use of a supercoiled plasmid template or a preopened linear template.

AB - NTRC is a prokaryotic enhancer-binding protein that activates transcription by σ54-holoenzyme. NTRC has an ATPase activity that is required for transcriptional activation, specifically for isomerization of closed complexes between σ54-holoenzyme and a promoter to open complexes. In the absence of ATP hydrolysis, there is known to be a kinetic barrier to open complex formation (i.e., the reaction proceeds so slowly that the polymerase synthesizes essentially no transcripts even from a supercoiled template). We show here that open complex formation is also thermodynamically unfavorable. In the absence of ATP hydrolysis the position of equilibrium between closed and open complexes favors the closed ones. Use of linear templates with a region of heteroduplex around the transcriptional start site-'preopened' templates-does not bypass the requirement for either NTRC or ATP hydrolysis, providing evidence that the rate-limiting step in open complex formation does not lie in DNA strand denaturation per se. These results are in contrast to recent findings regarding the ATP requirement for initiation of transcription by eukaryotic RNA polymerase II; in the latter case, the ATP requirement is circumvented by use of a supercoiled plasmid template or a preopened linear template.

KW - ATPase

KW - enhancers

KW - enteric bacteria

KW - heteroduplex template

KW - open complexes

KW - σ

UR - http://www.scopus.com/inward/record.url?scp=0029166594&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029166594&partnerID=8YFLogxK

U2 - 10.1101/gad.9.16.2042

DO - 10.1101/gad.9.16.2042

M3 - Article

C2 - 7649482

AN - SCOPUS:0029166594

VL - 9

SP - 2042

EP - 2052

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 16

ER -