The band 3-rich membrane of llama erythrocytes: Studies on cell shape and the organization of membrane proteins

Jena K. Khodadad, Ronald S. Weinstein

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The erythrocyte membrane of the llama was characterized in comparison to that of the human. The llama erythrocyte was an elliptical disk that resisted shape alterations in hyperosmotic buffers and following metabolic depletion, both of which induce spiculation of the human red cell. Lysophosphatidylcholine incorporation produced minor serrations of the edge of the llama disk but no spicules, whereas human red cells became sphero-echinocytes. The polypeptide profiles in the membranes of the two species were similar, except for several noteworthy differences: a marked elevation in the relative content of band 3; the absence of membrane-bound band 6; and simpler glycoprotein pattern in the llama. The concentration of band 3 in llama was about two and a half to three times that in the human and intramembrane particles in the protoplasmic leaflet of freeze-fractured llama membrane were correspondingly increased. The selective solubilization of bands 1, 2 and 5 in low ionic strength buffer, and all of the peripheral proteins in high alkaline buffer were similar except for increased retention of ankyrin by the llama membrane. These data suggest a similar disposition of membrane proteins. The llama membrane was markedly resistant to the solubilization of integral proteins by the nonionic detergent, Triton X-100. This property and the general resistance to shape changes may be related to the high concentration of band 3.

Original languageEnglish (US)
Pages (from-to)161-171
Number of pages11
JournalThe Journal of Membrane Biology
Volume72
Issue number3
DOIs
StatePublished - Oct 1 1983
Externally publishedYes

Keywords

  • band 3
  • cell shape
  • deformability
  • erythrocytes
  • llama
  • membranes
  • organization
  • proteins

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

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