The biologic activity of mast cell granules. IV. The effect of complement depletion on rat cutaneous late phase reactions

R. F. Lemanske, K. Joiner, M. Kaliner

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Cutaneous late phase reactions (LPR) in rats can be induced by the intradermal injection of anti-IgE antibody or isolated rat peritoneal mast cell granules. Rat LPR are characterized by neutrophil-rich infiltrates at 2 to 8 hr followed by mononuclear cell-rich infiltrates thereafter. Rat Arthus reactions are histologically similar and are complement (C) dependent. To determine the importance of C in the pathogenesis of rat LPR compared with its role in Arthus reactions, rats were treated with cobra venom factor (CVF) (250 U/kg i.v.), and the effects of this treatment on total hemolytic complement (CH50), C3 titers, LPR, and Arthus reactions were assessed. CVF treatment produced profound decreases in both CH50 (from 197 ± 20 to < 1.0 U/ml) and C3 (from 44,240 ± 2840 to < 5 U/ml) titers after 6 hr, which persisted through at least 30 hr. The inflammatory intensity of heterologous reverse passive Arthus reactions was significantly decreased in CVF-treated animals. In contrast, the intensity of LPR was unaffected by CVF treatment. Therefore, although LPR and Arthus reactions share certain histologic characteristics, these similarities are not due to a mutual requirement for the presence of C.

Original languageEnglish (US)
Pages (from-to)1881-1884
Number of pages4
JournalJournal of Immunology
Volume130
Issue number4
StatePublished - Jan 1 1983
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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