The Campylobacter jejuni dps homologue is important for in vitro biofilm formation and cecal colonization of poultry and may serve as a protective antigen for vaccination

James R. Theoret, Kerry K. Cooper, Bereket Zekarias, Kenneth L. Roland, Bibiana Law, Roy Curtiss, Lynn A. Joens

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In this work, we investigated the Campylobacter jejuni dps (DNA binding protein from starved cells) gene for a role in biofilm formation and cecal colonization in poultry. In vitro biofilm formation assays were conducted with stationary-phase cells in cell culture plates under microaerophilic conditions. These studies demonstrated a significant (>50%) reduction in biofilm formation by the C. jejuni dps mutant compared to that by the wild-type strain. Studies in poultry also demonstrated the importance of the dps gene in host colonization by C. jejuni. Real-time PCR analysis of mRNA extracted from the cecal contents of poultry infected with wild-type C. jejuni indicated that the dps gene is upregulated 20-fold during poultry colonization. Cecal colonization was greater than 5 log CFU lower in chicks infected with the dps mutant than chicks infected with the wild-type C. jejuni strain. Moreover, the dps mutant failed to colonize 75% of the chicks following challenge with 105 CFU. Preliminary studies were conducted in chicks by parenteral vaccination with a recombinant Dps protein or through oral vaccination with a recombinant attenuated Salmonella enterica strain synthesizing the C. jejuni Dps protein. No reduction in C. jejuni was noted in chicks vaccinated with the parenteral recombinant protein, whereas, a 2.5-log-unit reduction of C. jejuni was achieved in chicks vaccinated with the attenuated Salmonella vector after homologous challenge. Taken together, this work demonstrated the importance of Dps for biofilm formation and poultry colonization, and the study also provides a basis for continued work using the Dps protein as a vaccine antigen when delivered through a Salmonella vaccine vector.

Original languageEnglish (US)
Pages (from-to)1426-1431
Number of pages6
JournalClinical and Vaccine Immunology
Volume19
Issue number9
DOIs
StatePublished - Sep 2012

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Poultry
Campylobacter jejuni
Biofilms
Vaccination
Antigens
Salmonella
Genes
Salmonella Vaccines
Recombinant proteins
Recombinant Proteins
DNA-Binding Proteins
Cell culture
Assays
Proteins
Vaccines
Salmonella enterica
In Vitro Techniques
Messenger RNA
Real-Time Polymerase Chain Reaction
Cell Culture Techniques

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

The Campylobacter jejuni dps homologue is important for in vitro biofilm formation and cecal colonization of poultry and may serve as a protective antigen for vaccination. / Theoret, James R.; Cooper, Kerry K.; Zekarias, Bereket; Roland, Kenneth L.; Law, Bibiana; Curtiss, Roy; Joens, Lynn A.

In: Clinical and Vaccine Immunology, Vol. 19, No. 9, 09.2012, p. 1426-1431.

Research output: Contribution to journalArticle

Theoret, James R. ; Cooper, Kerry K. ; Zekarias, Bereket ; Roland, Kenneth L. ; Law, Bibiana ; Curtiss, Roy ; Joens, Lynn A. / The Campylobacter jejuni dps homologue is important for in vitro biofilm formation and cecal colonization of poultry and may serve as a protective antigen for vaccination. In: Clinical and Vaccine Immunology. 2012 ; Vol. 19, No. 9. pp. 1426-1431.
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