The capillary number effect on cell viability in Microfluidic Elasto-Filtration devices for viable circulating tumor cell isolation

Cong Zhao, Wenjuan Ma, Xingsu Yu, Huifang Su, Zhenfeng Zhang, Yitshak Zohar, Yi Kuen Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

This paper reports a systematic study of the Capillary number (Ca) effect on cell viability in Microfluidic Elasto-Filtration (MEF) devices for ex vivo circulating tumor cell (CTC) isolation. CTC viability decreases with increasing Ca because of the higher hydrodynamic shear stress. CTC viability also decreases with increasing cell diameter to filter-pore size ratio, d, due to the larger interaction forces between cells and filter pores. The cell viability dependence on Ca can be well fitted by two distinct functions for two ranges of d, larger and smaller than 2.5. Together with previously reported Ca effects on CTC capture efficiency and depletion of white blood cells, a complete design rule for MEF chips is proposed, yielding 92% CTC capture efficiency, 4-log WBC depletion, and 85% CTC viability at an optimized Ca.

Original languageEnglish (US)
Title of host publicationTRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages488-491
Number of pages4
ISBN (Electronic)9781538627310
DOIs
StatePublished - Jul 26 2017
Event19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan, Province of China
Duration: Jun 18 2017Jun 22 2017

Other

Other19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
CountryTaiwan, Province of China
CityKaohsiung
Period6/18/176/22/17

Keywords

  • Capillary number
  • Circulating tumor cells
  • Microfluidic Elasto-Filtration
  • Viability

ASJC Scopus subject areas

  • Chemical Health and Safety
  • Instrumentation
  • Electrical and Electronic Engineering

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    Zhao, C., Ma, W., Yu, X., Su, H., Zhang, Z., Zohar, Y., & Lee, Y. K. (2017). The capillary number effect on cell viability in Microfluidic Elasto-Filtration devices for viable circulating tumor cell isolation. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 488-491). [7994092] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994092