The cell adhesion molecule Roughest depends on ßHeavy-spectrin during eye morphogenesis in Drosophila

Hyun Gwan Lee, Daniela Zarnescu, Bryce MacIver, Graham H. Thomas

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Cell junctions have both structural and morphogenetic roles, and contain complex mixtures of proteins whose interdependencies are still largely unknown. Junctions are also major signaling centers that signify correct integration into a tissue, and modulate cell survival. During Drosophila eye development, the activity of the immunoglobulin cell adhesion molecule Roughest (also known as Irregular chiasm C-roughest protein) mediates interommatidial cell (IOC) reorganization, leading to an apoptotic event that refines the retinal lattice. Roughest and the cadherin-based zonula adherens (ZA) are interdependent and both are modulated by the apical polarity determinant, Crumbs. Here we describe a novel relationship between the Crumbs partner β Heavy-spectrin (βH), the ZA and Roughest. Ectopic expression of the C-terminal segment 33 of β H (βH33) induces defects in retinal morphogenesis, resulting the preferential loss of IOC. This effect is associated with ZA disruption and Roughest displacement. In addition, loss-of-function karst and roughest mutations interact to cause a synergistic and catastrophic effect on retinal development. Finally, we show that βH coimmunoprecipitates with Roughest and that the distribution of Roughest protein is disrupted in karst mutant tissue. These results suggest that the apical spectrin membrane skeleton helps to coordinate the Cadherin-based ZA with Roughest-based morphogenesis.

Original languageEnglish (US)
Pages (from-to)277-285
Number of pages9
JournalJournal of Cell Science
Volume123
Issue number2
DOIs
StatePublished - Jan 15 2010

Keywords

  • Apoptosis
  • Cell adhesion
  • Crumbs
  • Drosophila
  • IrreC
  • Membrane skeleton
  • Roughes
  • Spectrin

ASJC Scopus subject areas

  • Cell Biology

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