The cloning and expression of a human creatine transporter

Ichiro Sora, Jeremy Richman, Giovanna Santoro, Hongbing Wei, Yu Wang, Todd Vanderah, Robert Horvath, Mike Nguyen, Sue Waite, William R. Roeske, Henry I. Yamamura

Research output: Contribution to journalArticlepeer-review

104 Scopus citations

Abstract

A human creatine transporter (hCRT-BS2M) cDNA clone was isolated from a human brainstem/spinal cord using a PCR and phage plaque hybridization based technique. This clone included an open reading frame of 1,905 base pairs(bp) within a 2,283bp cDNA. Northern blot hybridization detected the expression of corresponding mRNAs most prominently in the skeletal muscle, heart and kidney. Peptide sequence analysis of the hCRT-BS2M protein product revealed 12 putative transmembrane domains. The predicted protein sequence further demonstrates that the hCRT-BS2M has highly conserved amino acid identity with the other members of the sodium dependent plasma membrane transporter family. Transient expression of the hCRT-BS2M in COS-7 cells demonstrates sodium dependent [14C]creatine uptake with a KM value of 14.9 ± 3.0 μM (n=5) that is attenuated by creatine and selective structural analogues of creatine.

Original languageEnglish (US)
Pages (from-to)419-427
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume204
Issue number1
DOIs
StatePublished - Oct 15 1994

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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