The Cloning and Expression of a Human Creatine Transporter

I. Sora, J. Richman, G. Santoro, H. B. Wei, Y. Wang, Todd W Vanderah, R. Horvath, M. Nguyen, S. Waite, William R Roeske, H. I. Yamamura

Research output: Contribution to journalArticle

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Abstract

A human creatine transporter (hCRT-BS2M) cDNA clone was isolated from a human brainstem/spinal cord using a PCR and phage plaque hybridization based technique. This clone included an open reading frame of 1,905 base pairs(bp) within a 2,283bp cDNA. Northern blot hybridization detected the expression of corresponding mRNAs most prominently in the skeletal muscle, heart and kidney. Peptide sequence analysis of the hCRT-BS2M protein product revealed 12 putative transmembrane domains. The predicted protein sequence further demonstrates that the hCRT-BS2M has highly conserved amino acid identity with the other members of the sodium dependent plasma membrane transporter family. Transient expression of the hCRT-BS2M in COS-7 cells demonstrates sodium dependent [14C]creatine uptake with a KM value of 14.9 ± 3.0 μM (n=5) that is attenuated by creatine and selective structural analogues of creatine.

Original languageEnglish (US)
Pages (from-to)419-427
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume204
Issue number1
DOIs
StatePublished - Oct 15 1994

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Creatine
Cloning
Organism Cloning
Complementary DNA
Clone Cells
Sodium
Bacteriophages
Membrane Transport Proteins
COS Cells
Protein Sequence Analysis
Cell membranes
Base Pairing
Northern Blotting
Open Reading Frames
Brain Stem
Muscle
Spinal Cord
Skeletal Muscle
Proteins
Cell Membrane

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

The Cloning and Expression of a Human Creatine Transporter. / Sora, I.; Richman, J.; Santoro, G.; Wei, H. B.; Wang, Y.; Vanderah, Todd W; Horvath, R.; Nguyen, M.; Waite, S.; Roeske, William R; Yamamura, H. I.

In: Biochemical and Biophysical Research Communications, Vol. 204, No. 1, 15.10.1994, p. 419-427.

Research output: Contribution to journalArticle

Sora, I, Richman, J, Santoro, G, Wei, HB, Wang, Y, Vanderah, TW, Horvath, R, Nguyen, M, Waite, S, Roeske, WR & Yamamura, HI 1994, 'The Cloning and Expression of a Human Creatine Transporter', Biochemical and Biophysical Research Communications, vol. 204, no. 1, pp. 419-427. https://doi.org/10.1006/bbrc.1994.2475
Sora, I. ; Richman, J. ; Santoro, G. ; Wei, H. B. ; Wang, Y. ; Vanderah, Todd W ; Horvath, R. ; Nguyen, M. ; Waite, S. ; Roeske, William R ; Yamamura, H. I. / The Cloning and Expression of a Human Creatine Transporter. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 204, No. 1. pp. 419-427.
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