The cloning and sequence analysis of the rat serotonin-1A receptor gene

Yutaka Fujiwara, David L. Nelson, Kenichi Kashihara, Eva Varga, William R. Roeske, Henry I. Yamamura

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Abstract

Using a probe derived from the human 5-HT1A receptor gene (G-21), a strongly hybridizable clone was identified from a rat genomic DNA library. This clone (G21Y2) had an overall deduced amino acid sequence that was 88% homologous with that of the human 5-HT1A receptor. There was only an 18% and 19% amino acid homology with the rat 5-HT1C and 5-HT2 receptors, respectively. The G21Y2 appears to be an excellent candidate for the rat 5-HT1A receptor gene. As this manuscript was being prepared a report by Albert et al. (13) was published in which a probe to the hamster β2-adrenergic receptor was used to identify a rat genomic (Clone D) that demonstrated a sequence that was similar to G21Y2.

Original languageEnglish (US)
Pages (from-to)PL127-PL132
JournalLife Sciences
Volume47
Issue number22
DOIs
StatePublished - 1990

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Fujiwara, Y., Nelson, D. L., Kashihara, K., Varga, E., Roeske, W. R., & Yamamura, H. I. (1990). The cloning and sequence analysis of the rat serotonin-1A receptor gene. Life Sciences, 47(22), PL127-PL132. https://doi.org/10.1016/0024-3205(90)90445-W