Abstract
The cluster Re6Se8I63− has been shown to induce preferential cell death of a hepatic carcinoma cell line, thus becoming a promising anti-cancer drug. Whether this cluster induces acute hemolysis or if it interacts with albumin remains unclear. The effect of acute exposure of human red blood cells to different concentrations of the cluster with and without albumin is described. Red blood cells from healthy donors were isolated, diluted at 1% hematocrit and exposed to the cluster (25–150 µM) at 37 °C, under agitation. Hemolysis and morphology were analyzed at 1 and 24 h. The potential protection of 0.1% albumin was also evaluated. Exposition to therapeutic doses of the cluster did not induce acute hemolysis. Similar results were observed following 24 h of exposition, and albumin slightly reduced hemolysis levels. Furthermore, the cluster induced alteration in the morphology of red blood cells, and this was prevented by albumin. Together, these results indicate that the cluster Re6Se8I63− is not a hemolytic component and induces moderate morphological alterations of red blood cells at high doses, which are prevented by co-incubation with albumin. In conclusion, the cluster Re6Se8I63− could be intravenously administered in animals at therapeutic doses for in vivostudies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1728-1735 |
| Number of pages | 8 |
| Journal | International Journal of Molecular Sciences |
| Volume | 16 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 13 2015 |
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Keywords
- Albumin
- Cluster
- Erythrocytes
- Hemolysis
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Organic Chemistry
- Spectroscopy
- Inorganic Chemistry
- Catalysis
- Molecular Biology
- Computer Science Applications
Cite this
The cluster [Re6Se8I6]3− induces low hemolysis of human erythrocytes in vitro : Protectives effect of albumin. / Rojas-Mancilla, Edgardo; Oyarce, Alexis; Verdugo, Viviana; Zheng, Zhiping; Ramírez-Tagle, Rodrigo.
In: International Journal of Molecular Sciences, Vol. 16, No. 1, 13.01.2015, p. 1728-1735.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The cluster [Re6Se8I6]3− induces low hemolysis of human erythrocytes in vitro
T2 - Protectives effect of albumin
AU - Rojas-Mancilla, Edgardo
AU - Oyarce, Alexis
AU - Verdugo, Viviana
AU - Zheng, Zhiping
AU - Ramírez-Tagle, Rodrigo
PY - 2015/1/13
Y1 - 2015/1/13
N2 - The cluster Re6Se8I63− has been shown to induce preferential cell death of a hepatic carcinoma cell line, thus becoming a promising anti-cancer drug. Whether this cluster induces acute hemolysis or if it interacts with albumin remains unclear. The effect of acute exposure of human red blood cells to different concentrations of the cluster with and without albumin is described. Red blood cells from healthy donors were isolated, diluted at 1% hematocrit and exposed to the cluster (25–150 µM) at 37 °C, under agitation. Hemolysis and morphology were analyzed at 1 and 24 h. The potential protection of 0.1% albumin was also evaluated. Exposition to therapeutic doses of the cluster did not induce acute hemolysis. Similar results were observed following 24 h of exposition, and albumin slightly reduced hemolysis levels. Furthermore, the cluster induced alteration in the morphology of red blood cells, and this was prevented by albumin. Together, these results indicate that the cluster Re6Se8I63− is not a hemolytic component and induces moderate morphological alterations of red blood cells at high doses, which are prevented by co-incubation with albumin. In conclusion, the cluster Re6Se8I63− could be intravenously administered in animals at therapeutic doses for in vivostudies.
AB - The cluster Re6Se8I63− has been shown to induce preferential cell death of a hepatic carcinoma cell line, thus becoming a promising anti-cancer drug. Whether this cluster induces acute hemolysis or if it interacts with albumin remains unclear. The effect of acute exposure of human red blood cells to different concentrations of the cluster with and without albumin is described. Red blood cells from healthy donors were isolated, diluted at 1% hematocrit and exposed to the cluster (25–150 µM) at 37 °C, under agitation. Hemolysis and morphology were analyzed at 1 and 24 h. The potential protection of 0.1% albumin was also evaluated. Exposition to therapeutic doses of the cluster did not induce acute hemolysis. Similar results were observed following 24 h of exposition, and albumin slightly reduced hemolysis levels. Furthermore, the cluster induced alteration in the morphology of red blood cells, and this was prevented by albumin. Together, these results indicate that the cluster Re6Se8I63− is not a hemolytic component and induces moderate morphological alterations of red blood cells at high doses, which are prevented by co-incubation with albumin. In conclusion, the cluster Re6Se8I63− could be intravenously administered in animals at therapeutic doses for in vivostudies.
KW - Albumin
KW - Cluster
KW - Erythrocytes
KW - Hemolysis
UR - http://www.scopus.com/inward/record.url?scp=84921275613&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84921275613&partnerID=8YFLogxK
U2 - 10.3390/ijms16011728
DO - 10.3390/ijms16011728
M3 - Article
VL - 16
SP - 1728
EP - 1735
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1422-0067
IS - 1
ER -