The copper-inducible cin operon encodes an unusual methionine-rich azurin-like protein and a pre-Q0 reductase in Pseudomonas putida KT2440

Davide Quaranta, Reid McCarty, Vahe Bandarian, Christopher Rensing

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The genome sequences of several pseudomonads have revealed a gene cluster containing genes for a two-component heavy metal histidine sensor kinase and response regulator upstream of cinA and cinQ, which we show herein to encode a copper-containing azurin-like protein and a pre-Q0 reductase, respectively. In the presence of copper, Pseudomonas putida KT2440 produces the CinA and CinQ proteins from a bicistronic mRNA. UV-visible spectra of CinA show features at 439, 581, and 719 nm, which is typical of the plastocyanin family of proteins. The redox potential of the protein was shown to be 456 ± 4 mV by voltametric titrations. Surprisingly, CinQ is a pyridine nucleotide-dependent nitrile oxidoreductase that catalyzes the conversion of pre-Q0 to pre-Q1 in the nucleoside queuosine biosynthetic pathway. Gene disruptions of cinA and cinQ did not lead to a significant increase in the copper sensitivity of P. putida KT2440 under the conditions tested. Possible roles of CinA and CinQ to help pseudomonads adapt and survive under prolonged copper stress are discussed.

Original languageEnglish (US)
Pages (from-to)5361-5371
Number of pages11
JournalJournal of Bacteriology
Volume189
Issue number14
DOIs
StatePublished - Jul 2007

Fingerprint

Azurin
Pseudomonas putida
Operon
Methionine
Copper
Oxidoreductases
Nucleoside Q
Proteins
Plastocyanin
Nitriles
Biosynthetic Pathways
Multigene Family
Heavy Metals
Nucleosides
Genes
Oxidation-Reduction
Nucleotides
Genome
Messenger RNA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

The copper-inducible cin operon encodes an unusual methionine-rich azurin-like protein and a pre-Q0 reductase in Pseudomonas putida KT2440. / Quaranta, Davide; McCarty, Reid; Bandarian, Vahe; Rensing, Christopher.

In: Journal of Bacteriology, Vol. 189, No. 14, 07.2007, p. 5361-5371.

Research output: Contribution to journalArticle

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