The crosslinking of nuclear protein to DNA using ionizing radiation

Anne E. Cress, Kirsten M. Kurath, Baldassarre Stea, G. Tim Bowden

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

DNA-protein complexes were generated in intact Chinese hamster ovary (CHO) cells by the use of ionizing radiation. The DNA-protein crosslinks, as measured by a filter-binding assay, occurred immediately following the irradiation, were produced in a dose-dependent manner and were reversible. The reversibility of the crosslinks in the intact cells was dependent upon general protein synthesis. Three proteins that were attached to DNA in unirradiated cells were analyzed according to the presence of DNA attached to the proteins before, during and after exposure to ionizing radiation. All three proteins contained more DNA reversibly attached to the proteins after exposure to 5 Gy ionizing radiation as compared to unirradiated cells. One of the proteins was increasingly attached to DNA using 2.5-50 Gy X-ray. These data suggest that the increased DNA-protein crosslinking observed with ionizing radiation may involve the increase in particular protein(s) crosslinked to DNA as well as an increase in the amount of DNA attached to specific proteins.

Original languageEnglish (US)
Pages (from-to)324-330
Number of pages7
JournalJournal of Cancer Research and Clinical Oncology
Volume116
Issue number4
DOIs
StatePublished - Jul 1990

Keywords

  • Cell culture
  • DNA damage
  • Ionizing radiation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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