The cyclin-dependent kinase 11 interacts with 14-3-3 proteins

Yongmei Feng, Wenqing Qi, Jesse Martinez, Mark A. Nelson

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Cyclin-dependent kinase 11 isoforms (CDK11) are members of the p34 cdc2 superfamily. They have been shown to play a role in RNA processing and apoptosis. In the present study, we investigate whether CDK11 interacts with 14-3-3 proteins. Our study shows that the putative 14-3-3 binding site (113-RHRSHS-118) within the N-terminal domain of CDK11p110 is functional. Endogenous CDK11p110 binds directly to 14-3-3 proteins and phosphorylation of the serine 118 within the RHRSHS motif seems to be required for the binding. Besides, CDK11p110 is capable of interacting with several different isoforms of 14-3-3 proteins both in vitro and in vivo. The interaction of 14-3-3 γ with CDK11p110 occurs throughout the entire cell cycle and reaches maximum at the G2/M phase. Interestingly, 14-3-3 γ shows strong interaction with N-terminal portion of caspase-cleaved CDK11p110 (CDK11p60) product at 48 h after Fas treatment, which correlates with the maximal cleavage level of CDK11p110 and the maximum activation level of CDK11 kinase activity during apoptosis. Collectively, these results suggest that CDK11 kinases could be regulated by interaction with 14-3-3 proteins during cell cycle and apoptosis.

Original languageEnglish (US)
Pages (from-to)1503-1509
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume331
Issue number4
DOIs
StatePublished - Jun 17 2005

Keywords

  • 14-3-3 proteins
  • Apoptosis
  • CDK11
  • Cell cycle

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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