The detection of mitotic and meiotic chromosome gain in the yeast Saccharomyces cerevisiae

Effects of methyl benzimidazol-2-yl carbamate, methyl methanesulfonate, ethyl methanesulfonate, dimethyl sulfoxide, propionitrile and cyclophosphamide monohydrate

S. G. Whittaker, S. F. Moser, D. H. Maloney, Walter W Piegorsch, M. A. Resnick, S. Fogel

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The diploid yeast strain BR1669 was used to study induction of mitotic and meiotic chromosome gain by selected chemical agents. The test relies on a gene dosage selection system in which hyperploidy is detected by the simultaneous increase in copy number of two alleles residing on the right arm of chromosome VIII: arg4-8 and cup1S (Rockmill and Fogel, 1988; Whittaker et al., 1988). Methyl methanesulfonate (MMS) induced mitotic, but not meiotic, chromosome gain. Methyl benzimidazol-2-yl carbamate (MBC) and ethyl methanesulfonate (EMS) induced both mitotic and meiotic chromosome gain. Propionitrile, a polar aprotic solvent, induced only mitotic chromosome gain; a reliable response was only achieved by overnight incubation of treated cultures at 0°C. MBC is postulated to act by binding directly to tubulin. The requirement for low-temperature incubation suggests that propionitrile also induces aneuploidy by perturbation of microtubular dynamics. The alkylating agents MMS and EMS probably induce recombination which might in turn perturb chromosome segregation. Cyclophosphamide monohydrate and dimethyl sulfoxide (DMSO) failed to induce mitotic or meiotic chromosome gain.

Original languageEnglish (US)
Pages (from-to)231-258
Number of pages28
JournalMutation Research/Genetic Toxicology
Volume242
Issue number3
DOIs
StatePublished - 1990
Externally publishedYes

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Ethyl Methanesulfonate
Methyl Methanesulfonate
Chromosomes
Dimethyl Sulfoxide
Yeast
Cyclophosphamide
Saccharomyces cerevisiae
Yeasts
Chromosome Segregation
Gene Dosage
Alkylating Agents
Aneuploidy
Tubulin
Carbamates
Diploidy
Genetic Recombination
carbendazim
propionitrile
Alleles
Genes

Keywords

  • Aneuploidy
  • Cyclophosphamide monohydrate
  • Dimethyl sulphoxide
  • Ethyl methanesulphonate
  • Meiosis
  • Methyl benzimidazol-2-yl carbamate
  • Methyl methanesulphonate
  • Mitosis
  • Propionitrile
  • Saccharomyces cerevisiae, chromosome gain

ASJC Scopus subject areas

  • Genetics
  • Toxicology
  • Medicine(all)

Cite this

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title = "The detection of mitotic and meiotic chromosome gain in the yeast Saccharomyces cerevisiae: Effects of methyl benzimidazol-2-yl carbamate, methyl methanesulfonate, ethyl methanesulfonate, dimethyl sulfoxide, propionitrile and cyclophosphamide monohydrate",
abstract = "The diploid yeast strain BR1669 was used to study induction of mitotic and meiotic chromosome gain by selected chemical agents. The test relies on a gene dosage selection system in which hyperploidy is detected by the simultaneous increase in copy number of two alleles residing on the right arm of chromosome VIII: arg4-8 and cup1S (Rockmill and Fogel, 1988; Whittaker et al., 1988). Methyl methanesulfonate (MMS) induced mitotic, but not meiotic, chromosome gain. Methyl benzimidazol-2-yl carbamate (MBC) and ethyl methanesulfonate (EMS) induced both mitotic and meiotic chromosome gain. Propionitrile, a polar aprotic solvent, induced only mitotic chromosome gain; a reliable response was only achieved by overnight incubation of treated cultures at 0°C. MBC is postulated to act by binding directly to tubulin. The requirement for low-temperature incubation suggests that propionitrile also induces aneuploidy by perturbation of microtubular dynamics. The alkylating agents MMS and EMS probably induce recombination which might in turn perturb chromosome segregation. Cyclophosphamide monohydrate and dimethyl sulfoxide (DMSO) failed to induce mitotic or meiotic chromosome gain.",
keywords = "Aneuploidy, Cyclophosphamide monohydrate, Dimethyl sulphoxide, Ethyl methanesulphonate, Meiosis, Methyl benzimidazol-2-yl carbamate, Methyl methanesulphonate, Mitosis, Propionitrile, Saccharomyces cerevisiae, chromosome gain",
author = "Whittaker, {S. G.} and Moser, {S. F.} and Maloney, {D. H.} and Piegorsch, {Walter W} and Resnick, {M. A.} and S. Fogel",
year = "1990",
doi = "10.1016/0165-1218(90)90089-K",
language = "English (US)",
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TY - JOUR

T1 - The detection of mitotic and meiotic chromosome gain in the yeast Saccharomyces cerevisiae

T2 - Effects of methyl benzimidazol-2-yl carbamate, methyl methanesulfonate, ethyl methanesulfonate, dimethyl sulfoxide, propionitrile and cyclophosphamide monohydrate

AU - Whittaker, S. G.

AU - Moser, S. F.

AU - Maloney, D. H.

AU - Piegorsch, Walter W

AU - Resnick, M. A.

AU - Fogel, S.

PY - 1990

Y1 - 1990

N2 - The diploid yeast strain BR1669 was used to study induction of mitotic and meiotic chromosome gain by selected chemical agents. The test relies on a gene dosage selection system in which hyperploidy is detected by the simultaneous increase in copy number of two alleles residing on the right arm of chromosome VIII: arg4-8 and cup1S (Rockmill and Fogel, 1988; Whittaker et al., 1988). Methyl methanesulfonate (MMS) induced mitotic, but not meiotic, chromosome gain. Methyl benzimidazol-2-yl carbamate (MBC) and ethyl methanesulfonate (EMS) induced both mitotic and meiotic chromosome gain. Propionitrile, a polar aprotic solvent, induced only mitotic chromosome gain; a reliable response was only achieved by overnight incubation of treated cultures at 0°C. MBC is postulated to act by binding directly to tubulin. The requirement for low-temperature incubation suggests that propionitrile also induces aneuploidy by perturbation of microtubular dynamics. The alkylating agents MMS and EMS probably induce recombination which might in turn perturb chromosome segregation. Cyclophosphamide monohydrate and dimethyl sulfoxide (DMSO) failed to induce mitotic or meiotic chromosome gain.

AB - The diploid yeast strain BR1669 was used to study induction of mitotic and meiotic chromosome gain by selected chemical agents. The test relies on a gene dosage selection system in which hyperploidy is detected by the simultaneous increase in copy number of two alleles residing on the right arm of chromosome VIII: arg4-8 and cup1S (Rockmill and Fogel, 1988; Whittaker et al., 1988). Methyl methanesulfonate (MMS) induced mitotic, but not meiotic, chromosome gain. Methyl benzimidazol-2-yl carbamate (MBC) and ethyl methanesulfonate (EMS) induced both mitotic and meiotic chromosome gain. Propionitrile, a polar aprotic solvent, induced only mitotic chromosome gain; a reliable response was only achieved by overnight incubation of treated cultures at 0°C. MBC is postulated to act by binding directly to tubulin. The requirement for low-temperature incubation suggests that propionitrile also induces aneuploidy by perturbation of microtubular dynamics. The alkylating agents MMS and EMS probably induce recombination which might in turn perturb chromosome segregation. Cyclophosphamide monohydrate and dimethyl sulfoxide (DMSO) failed to induce mitotic or meiotic chromosome gain.

KW - Aneuploidy

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KW - Meiosis

KW - Methyl benzimidazol-2-yl carbamate

KW - Methyl methanesulphonate

KW - Mitosis

KW - Propionitrile

KW - Saccharomyces cerevisiae, chromosome gain

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SN - 0165-1218

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