The detection of White Spot Syndrome Virus (WSSV) and Yellow Head Virus (YHV) in imported commodity shrimp

L. M. Nunan, B. T. Poulos, Donald V Lightner

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Transmission of exotic pathogens occurs through a variety of means, including migration with humans and animals, rapid transit by land, sea or air or through the shipment of infected frozen food products. White Spot Syndrome Virus (WSSV) and Yellow Head Virus (YHV) have caused mass mortalities of cultured shrimp in Asia beginning in 1992. In 1995, these viruses appeared for the first time in the Western Hemisphere causing high mortalities in farm reared shrimp in Texas, USA. The purpose of this study was to determine if WSSV and YHV are present in frozen shrimp products imported into the United States from Asia. Infectivity assays, transmission electron microscopy (TEM), and polymerase chain reaction (PCR) showed these viruses were detectable and infectious in frozen shrimp imports. Frozen shrimp were used to infect indicator shrimp (Penaeus stylirostris) which resulted in mortalities. The cause of these mortalities was determined by histology and TEM to be by YHV. PCR confirmed the presence of WSSV in the frozen, purchased products. The results from this study indicate that exotic shrimp pathogens can be transmitted via imported frozen products.

Original languageEnglish (US)
Pages (from-to)19-30
Number of pages12
JournalAquaculture
Volume160
Issue number1-2
DOIs
StatePublished - Jan 15 1998

Fingerprint

Gill-associated virus
White spot syndrome virus
products and commodities
commodity
virus
shrimp
frozen foods
transmission electron microscopy
mortality
polymerase chain reaction
Litopenaeus stylirostris
pathogen
viruses
pathogens
mass mortality
detection
infectivity
histology
imports
pathogenicity

Keywords

  • PCR
  • Penaeid shrimp
  • White Spot Syndrome Virus (WSSV)
  • Yellow Head Virus (YHV)

ASJC Scopus subject areas

  • Aquatic Science

Cite this

The detection of White Spot Syndrome Virus (WSSV) and Yellow Head Virus (YHV) in imported commodity shrimp. / Nunan, L. M.; Poulos, B. T.; Lightner, Donald V.

In: Aquaculture, Vol. 160, No. 1-2, 15.01.1998, p. 19-30.

Research output: Contribution to journalArticle

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