The diagnostic importance of species specific and cross-reactive components of Taenia solium, Echinococcus granulosus, and Hymenolepis nana.

T. Montenegro, R. H. Gilman, R. Castillo, V. Tsang, J. Brandt, A. Guevara, H. Sanabria, M. Verastegui, Charles R Sterling, E. Miranda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Sera from patients infected with Taenia solium, Hymenolepis nana and Echinococcus granulosus were tested against homologous and heterologous parasite antigens using an ELISA assay, and a high degree of cross-reactivity was verified. To identify polypeptides responsible for this cross reactivity, the Enzyme Linked Immunoelectro Transfer Blot (EITB) was used. Sera from infected patients with T.solium, H.nana, and E.granulosus were assessed against crude, ammonium sulphate precipitated (TSASP), and lentil-lectin purified antigens of T.solium and crude antigens of H.nana and E.granulosus. Several bands, recognized by sera from patients with T.solium, H.nana, and E.granulosus infections, were common to either two or all three cestodes. Unique reactive bands in H.nana were noted at 49 and 66 K-Da and in E.granulosus at 17-21 K-Da and at 27-32 K-Da. In the crude cysticercosis extract, a specific non glycoprotein band was present at 61-67 K-Da in addiction to specific glycoprotein bands of 50, 42, 24, 21, 18, 14, and 13 K-Da. None of the sera from patients with H.nana or E.granulosus infection cross reacted with these seven glycoprotein bands considered specific for T.solium infection.

Original languageEnglish (US)
Pages (from-to)327-334
Number of pages8
JournalRevista do Instituto de Medicina Tropical de Sao Paulo
Volume36
Issue number4
StatePublished - Jul 1994
Externally publishedYes

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Hymenolepis nana
Taenia solium
Echinococcus granulosus
Glycoproteins
Serum
Heterophile Antigens
Cysticercosis
Cestoda
Viral Tumor Antigens
Ammonium Sulfate
Cross Infection
Infection
Complex Mixtures
Parasites
Enzyme-Linked Immunosorbent Assay
Peptides
Enzymes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

The diagnostic importance of species specific and cross-reactive components of Taenia solium, Echinococcus granulosus, and Hymenolepis nana. / Montenegro, T.; Gilman, R. H.; Castillo, R.; Tsang, V.; Brandt, J.; Guevara, A.; Sanabria, H.; Verastegui, M.; Sterling, Charles R; Miranda, E.

In: Revista do Instituto de Medicina Tropical de Sao Paulo, Vol. 36, No. 4, 07.1994, p. 327-334.

Research output: Contribution to journalArticle

Montenegro, T, Gilman, RH, Castillo, R, Tsang, V, Brandt, J, Guevara, A, Sanabria, H, Verastegui, M, Sterling, CR & Miranda, E 1994, 'The diagnostic importance of species specific and cross-reactive components of Taenia solium, Echinococcus granulosus, and Hymenolepis nana.', Revista do Instituto de Medicina Tropical de Sao Paulo, vol. 36, no. 4, pp. 327-334.
Montenegro, T. ; Gilman, R. H. ; Castillo, R. ; Tsang, V. ; Brandt, J. ; Guevara, A. ; Sanabria, H. ; Verastegui, M. ; Sterling, Charles R ; Miranda, E. / The diagnostic importance of species specific and cross-reactive components of Taenia solium, Echinococcus granulosus, and Hymenolepis nana. In: Revista do Instituto de Medicina Tropical de Sao Paulo. 1994 ; Vol. 36, No. 4. pp. 327-334.
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