The differential effect of genetic variation on soluble CD14 levels in human plasma and milk

Stefano Guerra, I. Carla Lohman, Tricia D. LeVan, Anne L Wright, Fernando D. Martinez, Marilyn Halonen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Problem: The protein CD14 is a pattern recognition receptor for bacterial lipopolysaccharide (LPS). Whether genetic variation has the same influence on soluble CD14 (sCD14) levels in human plasma and milk remains to be determined. Method of study: We measured sCD14 levels in plasma during pregnancy (n = 196) and in milk in the postpartum (n = 152) for women genotyped for the single nucleotide polymorphisms (SNPs) at positions -1619, -550, and -159 from the transcription start site of the CD14 gene. Results: Plasma- and milk-sCD14 levels differed significantly both by CD14/-1619 and CD14/-550 genotypes and by haplotypes. Most interestingly, sCD14 levels were regulated differentially by the same genetic variants in plasma and milk, with the CD14/-550T allele and the corresponding are ATC haplotype associated with high sCD14 in milk but low sCD14 in plasma. A correlation between sCD14 levels in plasma and milk was absent (r = 0.091, P = NS). Conclusions: Our findings suggest the existence of cell-specific regulation mechanisms of CD14 gene expression.

Original languageEnglish (US)
Pages (from-to)204-211
Number of pages8
JournalAmerican Journal of Reproductive Immunology
Volume52
Issue number3
StatePublished - Sep 2004

Fingerprint

Human Milk
Milk
Haplotypes
Pattern Recognition Receptors
Transcription Initiation Site
Postpartum Period
Single Nucleotide Polymorphism
Lipopolysaccharides
Alleles
Genotype
Gene Expression
Pregnancy
Genes
Proteins

Keywords

  • CD14 Antigen
  • Gene regulation
  • Human milk
  • Lipopolysaccharide
  • Pregnancy
  • Single Nucleotide polymorphism

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

Cite this

The differential effect of genetic variation on soluble CD14 levels in human plasma and milk. / Guerra, Stefano; Lohman, I. Carla; LeVan, Tricia D.; Wright, Anne L; Martinez, Fernando D.; Halonen, Marilyn.

In: American Journal of Reproductive Immunology, Vol. 52, No. 3, 09.2004, p. 204-211.

Research output: Contribution to journalArticle

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AU - Halonen, Marilyn

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