The direct in vitro effect of insulin-like growth factors (IGFs) on normal bovine mammary cell proliferation and production of IGF binding proteins

M. F. McGrath, Robert J Collier, D. R. Clemmons, W. H. Busby, C. A. Sweeny, G. G. Krivi

Research output: Contribution to journalArticle

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Abstract

Mammary epithelial cells isolated from pregnant, nonlactating heifers were grown in vitro using collagen substrates. Using these systems, the truncated form of insulin growth factor-1 (IGF-1) (des-3-IGF-1), IGF-1, and IGF-2 all stimulated a significant (0.5 to 1 fold) increase in cell proliferation (des-3-IGF-1 > IGF-1 > IGF-2). When grown in media containing serum plus IGF-1, normal bovine mammary cells also produced and secreted at least four species of IGF-binding protein (IGFBP) ranging from 21K to 48K (as demonstrated by ligand blot analysis). However, cells grown in serum free media secreted detectable quantities of only 2 major forms of IGFBP of 34K and 48K. Using immunoblot analysis, these proteins were identified as IGFBP-2 and IGFBP-3, respectively. Both proteins were inducible by the addition of IGF to the serum free media (relative potency; IGF-1 > des-3-IGF-1 > IGF-2). Using RIA analysis, bovine mammary cells cultured in the presence of IGF-1 produced 20-25 ng/ml IGFBP-2 compared to control cultures which secrete approximately 1.0 ng/ml. Cells exposed to des-3-IGF-1 produced 40-60% less IGFBP-2 whereas insulin and IGF-2 did not stimulate significant IGFBP-2 production. These data indicate that normal bovine mammary cells secrete IGFBP-2 and IGFBP-3. This secretion is stimulated by IGF-1 and des-3-IGF-1 suggesting a mechanism for regulating local IGF activity.

Original languageEnglish (US)
Pages (from-to)671-678
Number of pages8
JournalEndocrinology
Volume129
Issue number2
StatePublished - Aug 1991
Externally publishedYes

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Insulin-Like Growth Factor Binding Proteins
Somatomedins
Breast
Cell Proliferation
Insulin-Like Growth Factor Binding Protein 2
In Vitro Techniques
Insulin-Like Growth Factor Binding Protein 3
Serum-Free Culture Media
Insulin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

The direct in vitro effect of insulin-like growth factors (IGFs) on normal bovine mammary cell proliferation and production of IGF binding proteins. / McGrath, M. F.; Collier, Robert J; Clemmons, D. R.; Busby, W. H.; Sweeny, C. A.; Krivi, G. G.

In: Endocrinology, Vol. 129, No. 2, 08.1991, p. 671-678.

Research output: Contribution to journalArticle

McGrath, M. F. ; Collier, Robert J ; Clemmons, D. R. ; Busby, W. H. ; Sweeny, C. A. ; Krivi, G. G. / The direct in vitro effect of insulin-like growth factors (IGFs) on normal bovine mammary cell proliferation and production of IGF binding proteins. In: Endocrinology. 1991 ; Vol. 129, No. 2. pp. 671-678.
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