The distribution of la antigens of the H-2 complex on lymph node cells by immunoferritin labelling

Jane S. Schultz, Jeffrey A Frelinger, Sun Kee Kim, Donald C. Shreffler

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The distribution of Ia antigens on the surfaces of lymph node lymphocytes of several mouse strains was investigated using indirect immunoferritin labeling and electron microscopy. The immunoferritin labeling results agreed with results of cytotoxic tests in strain distribution of reactivity, proportion of cells showing label, and cell populations reacting. Capping was induced by increased incubation temperature but conditions for Ia antigen mobilization varied somewhat between the two anti-Ia antisera employed. Uncapped specimens generally showed a denser, more evenly distributed antigen coating than is the case for H-2 antigens labeled by the same indirect immunoferritin method.

Original languageEnglish (US)
Pages (from-to)125-134
Number of pages10
JournalCellular Immunology
Volume16
Issue number1
DOIs
StatePublished - 1975
Externally publishedYes

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H-2 Antigens
Histocompatibility Antigens Class II
Lymph Nodes
Immune Sera
Electron Microscopy
Lymphocytes
Antigens
Temperature
Population

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

The distribution of la antigens of the H-2 complex on lymph node cells by immunoferritin labelling. / Schultz, Jane S.; Frelinger, Jeffrey A; Kim, Sun Kee; Shreffler, Donald C.

In: Cellular Immunology, Vol. 16, No. 1, 1975, p. 125-134.

Research output: Contribution to journalArticle

Schultz, Jane S. ; Frelinger, Jeffrey A ; Kim, Sun Kee ; Shreffler, Donald C. / The distribution of la antigens of the H-2 complex on lymph node cells by immunoferritin labelling. In: Cellular Immunology. 1975 ; Vol. 16, No. 1. pp. 125-134.
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