The distribution of la antigens of the H-2 complex on lymph node cells by immunoferritin labelling

Jane S. Schultz, Jeffrey A. Frelinger, Sun Kee Kim, Donald C. Shreffler

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The distribution of Ia antigens on the surfaces of lymph node lymphocytes of several mouse strains was investigated using indirect immunoferritin labeling and electron microscopy. The immunoferritin labeling results agreed with results of cytotoxic tests in strain distribution of reactivity, proportion of cells showing label, and cell populations reacting. Capping was induced by increased incubation temperature but conditions for Ia antigen mobilization varied somewhat between the two anti-Ia antisera employed. Uncapped specimens generally showed a denser, more evenly distributed antigen coating than is the case for H-2 antigens labeled by the same indirect immunoferritin method.

Original languageEnglish (US)
Pages (from-to)125-134
Number of pages10
JournalCellular Immunology
Volume16
Issue number1
DOIs
StatePublished - Mar 1975
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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