The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1

Magdalene So, Fred Heffron, Brian J. McCarthy

Research output: Contribution to journalArticle

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Abstract

Restriction endonuclease subclones of the Escherichia coli gene encoding the heat stable (ST) toxin exhibit a stem and loop structure similar to those seen in many procaryotic transposons. An EcoRI DNA fragment encoding tetra-cycline (Tc) resistance but no transposition functions was spliced into the ST gene in one of these subclones. By monitoring Tcr, we were able to show that the ST gene transposes. Restriction and DNA sequence data strongly suggest that the ST transposon, Tn1681, is flanked by inverted repeats of IS1.

Original languageEnglish (US)
Pages (from-to)453-456
Number of pages4
JournalNature
Volume277
Issue number5696
DOIs
StatePublished - Dec 1 1979
Externally publishedYes

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