Abstract
The role of C-kinase in the activation of human polymorphonuclear leukocytes has been examined using H-7, a recently described C-kinase inhibitor. We found that H-7 will inhibit PMN superoxide anion release in response to the tumor promotor phorbol myristate acetate and the calcium ionophore A23187. In contrast, no inhibition by H-7 was seen for PMN superoxide release stimulated by the chemotactic peptide FMLP. H-7 did not inhibit PMN NADPH oxidase activity or PMN degranulation by any stimulant, but it reversed a phorbol ester-induced inhibition of granule release by FMLP. The results show that H-7 differentially affects the PMN functional events of secretion and superoxide release and suggests that an H-7 inhibitable C-kinase 1) is not involved in chemotactic peptide induced activation of PMN and 2) may not regulate stimulus induced PMN degranulation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 441-446 |
| Number of pages | 6 |
| Journal | Journal of Leukocyte Biology |
| Volume | 41 |
| Issue number | 5 |
| State | Published - 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
Cite this
The effect of a protein kinase C inhibitor, H-7, on human neutrophil oxidative burst and degranulation. / Berkow, R. L.; Dodson, R. W.; Kraft, Andrew.
In: Journal of Leukocyte Biology, Vol. 41, No. 5, 1987, p. 441-446.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect of a protein kinase C inhibitor, H-7, on human neutrophil oxidative burst and degranulation
AU - Berkow, R. L.
AU - Dodson, R. W.
AU - Kraft, Andrew
PY - 1987
Y1 - 1987
N2 - The role of C-kinase in the activation of human polymorphonuclear leukocytes has been examined using H-7, a recently described C-kinase inhibitor. We found that H-7 will inhibit PMN superoxide anion release in response to the tumor promotor phorbol myristate acetate and the calcium ionophore A23187. In contrast, no inhibition by H-7 was seen for PMN superoxide release stimulated by the chemotactic peptide FMLP. H-7 did not inhibit PMN NADPH oxidase activity or PMN degranulation by any stimulant, but it reversed a phorbol ester-induced inhibition of granule release by FMLP. The results show that H-7 differentially affects the PMN functional events of secretion and superoxide release and suggests that an H-7 inhibitable C-kinase 1) is not involved in chemotactic peptide induced activation of PMN and 2) may not regulate stimulus induced PMN degranulation.
AB - The role of C-kinase in the activation of human polymorphonuclear leukocytes has been examined using H-7, a recently described C-kinase inhibitor. We found that H-7 will inhibit PMN superoxide anion release in response to the tumor promotor phorbol myristate acetate and the calcium ionophore A23187. In contrast, no inhibition by H-7 was seen for PMN superoxide release stimulated by the chemotactic peptide FMLP. H-7 did not inhibit PMN NADPH oxidase activity or PMN degranulation by any stimulant, but it reversed a phorbol ester-induced inhibition of granule release by FMLP. The results show that H-7 differentially affects the PMN functional events of secretion and superoxide release and suggests that an H-7 inhibitable C-kinase 1) is not involved in chemotactic peptide induced activation of PMN and 2) may not regulate stimulus induced PMN degranulation.
UR - http://www.scopus.com/inward/record.url?scp=0023219933&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023219933&partnerID=8YFLogxK
M3 - Article
C2 - 3033114
AN - SCOPUS:0023219933
VL - 41
SP - 441
EP - 446
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
SN - 0741-5400
IS - 5
ER -