The effect of bacterial challenge on ferritin regulation in the yellow fever mosquito, Aedes aegypti

Dawn L. Geiser, Guoli Zhou, Jonathan J. Mayo, Joy Winzerling

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Secreted ferritin is the major iron storage and transport protein in insects. Here, we characterize the message and protein expression profiles of yellow fever mosquito (Aedes aegypti) ferritin heavy chain homologue (HCH) and light chain homologue (LCH) subunits in response to iron and bacterial challenge. In vivo experiments demonstrated tissue-specific regulation of HCH and LCH expression over time post-blood meal (PBM). Transcriptional regulation of HCH and LCH was treatment specific, with differences in regulation for naïve versus mosquitoes challenged with heat-killed bacteria (HKB). Translational regulation by iron regulatory protein (IRP) binding activity for the iron-responsive element (IRE) was tissue-specific and time-dependent PBM. However, mosquitoes challenged with HKB showed little change in IRP/IRE binding activity compared to naïve animals. The changes in ferritin regulation and expression in vivo were confirmed with in vitro studies. We challenged mosquitoes with HKB followed by a blood meal to determine the effects on ferritin expression, and demonstrate a synergistic, time-dependent regulation of expression for HCH and LCH.

Original languageEnglish (US)
Pages (from-to)601-619
Number of pages19
JournalInsect Science
Volume20
Issue number5
DOIs
StatePublished - Oct 2013

Fingerprint

yellow fever
Yellow Fever
Aedes
ferritin
Ferritins
Aedes aegypti
Culicidae
mosquito
Iron
Iron-Regulatory Proteins
iron
Meals
Light
Bacteria
Blood
Hot Temperature
blood meal
protein
blood
regulatory proteins

Keywords

  • Aedes aegypti
  • Bacteria
  • Ferritin
  • Iron
  • IRP
  • Mosquito

ASJC Scopus subject areas

  • Insect Science
  • Ecology, Evolution, Behavior and Systematics
  • Agronomy and Crop Science
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The effect of bacterial challenge on ferritin regulation in the yellow fever mosquito, Aedes aegypti. / Geiser, Dawn L.; Zhou, Guoli; Mayo, Jonathan J.; Winzerling, Joy.

In: Insect Science, Vol. 20, No. 5, 10.2013, p. 601-619.

Research output: Contribution to journalArticle

Geiser, Dawn L. ; Zhou, Guoli ; Mayo, Jonathan J. ; Winzerling, Joy. / The effect of bacterial challenge on ferritin regulation in the yellow fever mosquito, Aedes aegypti. In: Insect Science. 2013 ; Vol. 20, No. 5. pp. 601-619.
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