Abstract
The effect of choline deficiency on the kinetic properties of the microsomal enzyme UDP-glucuronyltransferase (EC2.4.1.17) was investigated in rats. Animals fed choline-deficient diets, as compared with animals fed a choline-replete diet or standard laboratory chow, showed almost a three-fold increase in enzyme activity when the enzyme was assayed at physiological concentrations of UDP-glucuronic acid (0.25 mM). The increase in activity appeared to be due to an enhanced affinity of the enzyme for UDP-glucuronic acid rather than to an increase in the amount of enzyme. These data indicate that the kinetic properties of tightly bound membrane enzymes are altered by a dietary change that is known to cause liver disease in the rat.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 418-424 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 114 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 18 1983 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Boyer, T. D., & Zakim, D. (1983). The effect of choline deficiency on the activity of a phosphatidylcholine-requiring enzyme: Activity and properties of UDP-glucuronyltransferase in choline-deficient rats. Biochemical and Biophysical Research Communications, 114(1), 418-424. https://doi.org/10.1016/0006-291X(83)91643-1