TY - JOUR
T1 - The effect of choline deficiency on the activity of a phosphatidylcholine-requiring enzyme
T2 - Activity and properties of UDP-glucuronyltransferase in choline-deficient rats
AU - Boyer, Thomas David
AU - Zakim, David
PY - 1983/7/18
Y1 - 1983/7/18
N2 - The effect of choline deficiency on the kinetic properties of the microsomal enzyme UDP-glucuronyltransferase (EC2.4.1.17) was investigated in rats. Animals fed choline-deficient diets, as compared with animals fed a choline-replete diet or standard laboratory chow, showed almost a three-fold increase in enzyme activity when the enzyme was assayed at physiological concentrations of UDP-glucuronic acid (0.25 mM). The increase in activity appeared to be due to an enhanced affinity of the enzyme for UDP-glucuronic acid rather than to an increase in the amount of enzyme. These data indicate that the kinetic properties of tightly bound membrane enzymes are altered by a dietary change that is known to cause liver disease in the rat.
AB - The effect of choline deficiency on the kinetic properties of the microsomal enzyme UDP-glucuronyltransferase (EC2.4.1.17) was investigated in rats. Animals fed choline-deficient diets, as compared with animals fed a choline-replete diet or standard laboratory chow, showed almost a three-fold increase in enzyme activity when the enzyme was assayed at physiological concentrations of UDP-glucuronic acid (0.25 mM). The increase in activity appeared to be due to an enhanced affinity of the enzyme for UDP-glucuronic acid rather than to an increase in the amount of enzyme. These data indicate that the kinetic properties of tightly bound membrane enzymes are altered by a dietary change that is known to cause liver disease in the rat.
UR - http://www.scopus.com/inward/record.url?scp=0020504888&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020504888&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(83)91643-1
DO - 10.1016/0006-291X(83)91643-1
M3 - Article
C2 - 6411083
AN - SCOPUS:0020504888
VL - 114
SP - 418
EP - 424
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -