The effect of isoprene on the properties of spinach thylakoids and phosphatidylcholine liposomes

B. A. Logan, T. J. Anchordoquy, Russell Monson, R. S. Pan

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Isoprene is emitted from the leaves of some plants. It was recently reported that exogenous isoprene delays the onset of leaf damage during controlled increases in leaf temperature (Singsaas et al. Plant Physiology 115: 1413-1420 [1997]). Thylakoid membranes are presumed to be the site of action based upon isoprene's hydrophobicity, production in chloroplasts, and effect upon chlorophyll fluorescence at high temperatures. In an attempt to discern the mechanistic basis for isoprene's thermoprotective role, we studied the effect of exogenous isoprene on the peroxidation, permeability, and stability of spinach thylakoids and phosphatidylcholine liposomes. Isoprene, supplied at either 18 or 21 μL L-1, had no effect upon the rate of liposome peroxidation in the presence of a hydroxyl radical-generating system. Isoprene also did not affect liposome peroxidation at high temperatures. Neither the proton permeability of thylakoids nor the leakage of a fluorescent probe from liposomes was influenced by exogenous isoprene, when measured at several temperatures. Isoprene did not affect the stability of thylakoid membrane proteins during a temperature increase, as shown by differential scanning calorimetry. Therefore, despite the use of a variety of techniques to investigate fundamental membrane parameters, we were unable to demonstrate an effect of isoprene.

Original languageEnglish (US)
Pages (from-to)602-606
Number of pages5
JournalPlant Biology
Volume1
Issue number6
StatePublished - Nov 1999
Externally publishedYes

Fingerprint

lipid bodies
isoprene
spinach
thylakoids
phosphatidylcholines
peroxidation
membrane
temperature
permeability
effect
leaves
calorimetry
plant physiology
hydrophobicity
hydroxyl radicals
hydroxyl radical
differential scanning calorimetry
chloroplast
membrane proteins
protons

Keywords

  • Differential scanning calorimetry
  • Isoprene
  • Lipid peroxidation
  • Liposomes
  • Membrane permeability
  • Thylakoids

ASJC Scopus subject areas

  • Plant Science

Cite this

The effect of isoprene on the properties of spinach thylakoids and phosphatidylcholine liposomes. / Logan, B. A.; Anchordoquy, T. J.; Monson, Russell; Pan, R. S.

In: Plant Biology, Vol. 1, No. 6, 11.1999, p. 602-606.

Research output: Contribution to journalArticle

Logan, B. A. ; Anchordoquy, T. J. ; Monson, Russell ; Pan, R. S. / The effect of isoprene on the properties of spinach thylakoids and phosphatidylcholine liposomes. In: Plant Biology. 1999 ; Vol. 1, No. 6. pp. 602-606.
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