The effect of turmeric extracts on inflammatory mediator production

Robert Clark Lantz, G. J. Chen, A. M. Solyom, S. D. Jolad, B. N. Timmermann

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Abstract

Major compounds of several commonly used botanicals, including turmeric, have been purported to have anti-inflammatory actions. In order to test the anti-inflammatory activity of compounds isolated from rhizomes of Curcuma longa L. (Zingiberaceae), we have established an in vitro test system. HL-60 cells were differentiated and exposed to lipopolysaccharide (LPS) from Escherichia coli (1 μg/ml) in the presence or absence of botanical compounds for 24 h. Supernatants were collected and analyzed for the production of tumor necrosis factor alpha (TNF-α) and prostaglandin E2 (PGE2) using standard ELISA assays. Water-soluble extracts were not cytotoxic and did not exhibit biological activity. Organic extracts of turmeric were cytotoxic only at concentrations above 50 μg/ml. Crude organic extracts of turmeric were capable of inhibiting LPS-induced TNF-α (IC50 value=15.2 μg/ml) and PGE2 (IC50 value=0.92 μg/ml) production. Purified curcumin was more active than either demethoxy- or bisdemethoxycurcumin. Fractions and subfractions of turmeric extracts collected via preparative HPLC had differing biological activity, ranging from no activity to IC50 values of <1 μg/ml. For some fractions, subfractionation resulted in a loss of activity, indicating interaction of the compounds within the fraction to produce an anti-inflammatory effect. A combination of several of the fractions that contain the turmeric oils was more effective than the curcuminoids at inhibiting PGE2. While curcumin inhibited COX-2 expression, turmeric oils had no effect on levels of COX-2 mRNA.

Original languageEnglish (US)
Pages (from-to)445-452
Number of pages8
JournalPhytomedicine
Volume12
Issue number6-7
DOIs
Publication statusPublished - Jun 15 2005

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Keywords

  • Anti-inflammatory
  • Curcuma longa L. (Zingiberaceae)
  • Cytokines
  • PGE
  • Prostaglandins
  • TNF-α
  • Turmeric

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Lantz, R. C., Chen, G. J., Solyom, A. M., Jolad, S. D., & Timmermann, B. N. (2005). The effect of turmeric extracts on inflammatory mediator production. Phytomedicine, 12(6-7), 445-452. https://doi.org/10.1016/j.phymed.2003.12.011