Abstract
Major compounds of several commonly used botanicals, including turmeric, have been purported to have anti-inflammatory actions. In order to test the anti-inflammatory activity of compounds isolated from rhizomes of Curcuma longa L. (Zingiberaceae), we have established an in vitro test system. HL-60 cells were differentiated and exposed to lipopolysaccharide (LPS) from Escherichia coli (1 μg/ml) in the presence or absence of botanical compounds for 24 h. Supernatants were collected and analyzed for the production of tumor necrosis factor alpha (TNF-α) and prostaglandin E2 (PGE2) using standard ELISA assays. Water-soluble extracts were not cytotoxic and did not exhibit biological activity. Organic extracts of turmeric were cytotoxic only at concentrations above 50 μg/ml. Crude organic extracts of turmeric were capable of inhibiting LPS-induced TNF-α (IC50 value=15.2 μg/ml) and PGE2 (IC50 value=0.92 μg/ml) production. Purified curcumin was more active than either demethoxy- or bisdemethoxycurcumin. Fractions and subfractions of turmeric extracts collected via preparative HPLC had differing biological activity, ranging from no activity to IC50 values of <1 μg/ml. For some fractions, subfractionation resulted in a loss of activity, indicating interaction of the compounds within the fraction to produce an anti-inflammatory effect. A combination of several of the fractions that contain the turmeric oils was more effective than the curcuminoids at inhibiting PGE2. While curcumin inhibited COX-2 expression, turmeric oils had no effect on levels of COX-2 mRNA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 445-452 |
| Number of pages | 8 |
| Journal | Phytomedicine |
| Volume | 12 |
| Issue number | 6-7 |
| DOIs | |
| State | Published - Jun 15 2005 |
Fingerprint
Keywords
- Anti-inflammatory
- Curcuma longa L. (Zingiberaceae)
- Cytokines
- PGE
- Prostaglandins
- TNF-α
- Turmeric
ASJC Scopus subject areas
- Plant Science
- Drug Discovery
- Organic Chemistry
- Pharmacology
Cite this
The effect of turmeric extracts on inflammatory mediator production. / Lantz, Robert Clark; Chen, G. J.; Solyom, A. M.; Jolad, S. D.; Timmermann, B. N.
In: Phytomedicine, Vol. 12, No. 6-7, 15.06.2005, p. 445-452.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect of turmeric extracts on inflammatory mediator production
AU - Lantz, Robert Clark
AU - Chen, G. J.
AU - Solyom, A. M.
AU - Jolad, S. D.
AU - Timmermann, B. N.
PY - 2005/6/15
Y1 - 2005/6/15
N2 - Major compounds of several commonly used botanicals, including turmeric, have been purported to have anti-inflammatory actions. In order to test the anti-inflammatory activity of compounds isolated from rhizomes of Curcuma longa L. (Zingiberaceae), we have established an in vitro test system. HL-60 cells were differentiated and exposed to lipopolysaccharide (LPS) from Escherichia coli (1 μg/ml) in the presence or absence of botanical compounds for 24 h. Supernatants were collected and analyzed for the production of tumor necrosis factor alpha (TNF-α) and prostaglandin E2 (PGE2) using standard ELISA assays. Water-soluble extracts were not cytotoxic and did not exhibit biological activity. Organic extracts of turmeric were cytotoxic only at concentrations above 50 μg/ml. Crude organic extracts of turmeric were capable of inhibiting LPS-induced TNF-α (IC50 value=15.2 μg/ml) and PGE2 (IC50 value=0.92 μg/ml) production. Purified curcumin was more active than either demethoxy- or bisdemethoxycurcumin. Fractions and subfractions of turmeric extracts collected via preparative HPLC had differing biological activity, ranging from no activity to IC50 values of <1 μg/ml. For some fractions, subfractionation resulted in a loss of activity, indicating interaction of the compounds within the fraction to produce an anti-inflammatory effect. A combination of several of the fractions that contain the turmeric oils was more effective than the curcuminoids at inhibiting PGE2. While curcumin inhibited COX-2 expression, turmeric oils had no effect on levels of COX-2 mRNA.
AB - Major compounds of several commonly used botanicals, including turmeric, have been purported to have anti-inflammatory actions. In order to test the anti-inflammatory activity of compounds isolated from rhizomes of Curcuma longa L. (Zingiberaceae), we have established an in vitro test system. HL-60 cells were differentiated and exposed to lipopolysaccharide (LPS) from Escherichia coli (1 μg/ml) in the presence or absence of botanical compounds for 24 h. Supernatants were collected and analyzed for the production of tumor necrosis factor alpha (TNF-α) and prostaglandin E2 (PGE2) using standard ELISA assays. Water-soluble extracts were not cytotoxic and did not exhibit biological activity. Organic extracts of turmeric were cytotoxic only at concentrations above 50 μg/ml. Crude organic extracts of turmeric were capable of inhibiting LPS-induced TNF-α (IC50 value=15.2 μg/ml) and PGE2 (IC50 value=0.92 μg/ml) production. Purified curcumin was more active than either demethoxy- or bisdemethoxycurcumin. Fractions and subfractions of turmeric extracts collected via preparative HPLC had differing biological activity, ranging from no activity to IC50 values of <1 μg/ml. For some fractions, subfractionation resulted in a loss of activity, indicating interaction of the compounds within the fraction to produce an anti-inflammatory effect. A combination of several of the fractions that contain the turmeric oils was more effective than the curcuminoids at inhibiting PGE2. While curcumin inhibited COX-2 expression, turmeric oils had no effect on levels of COX-2 mRNA.
KW - Anti-inflammatory
KW - Curcuma longa L. (Zingiberaceae)
KW - Cytokines
KW - PGE
KW - Prostaglandins
KW - TNF-α
KW - Turmeric
UR - http://www.scopus.com/inward/record.url?scp=19944361837&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19944361837&partnerID=8YFLogxK
U2 - 10.1016/j.phymed.2003.12.011
DO - 10.1016/j.phymed.2003.12.011
M3 - Article
C2 - 16008121
AN - SCOPUS:19944361837
VL - 12
SP - 445
EP - 452
JO - Phytomedicine
JF - Phytomedicine
SN - 0944-7113
IS - 6-7
ER -