In many tissues, the level of cytoplasmic calcium mediates cell function. Since cytoplasmic calcium is often maintained at a low level by active calcium extrusion, we examined whether calcium-stimulated ATPase is present in the rabbit ciliary epithelium. A technique was developed to measure calcium-stimulated ATPase in a partially enriched plasma membrane preparation. The enhancement of Na,K-ATPase activity was used to indicate the enrichment of plasma membrane. Marked stimulation of ATPase activity by calcium was observed over a range of calcium concentrations (10-8 to 10-3 M). The calcium concentration necessary to elicit half-maximal ATPase activity was 10-6 M, which is similar to that reported for other membrane preparations. Calcium-stimulated ATPase activity was significantly inhibited in the presence of low concentrations of sodium orthovanadate. The inhibitory influence of vanadate was examined over a range of vanadate concentrations (10-8 to 10-3 M). The vanadate concentration needed to produce half-maximal inhibition of calcium-stimulated ATPase was 2 x 10-6 M. These studies show that calcium-stimulated ATPase inhibition can occur, in vitro, at very low levels of vanadate; it is possible that this might contribute to the chain of events which results in the lowering of aqueous humor secretion reported in vanadate-treated rabbits.
|Original language||English (US)|
|Number of pages||5|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - Dec 1 1988|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience