The effect of vanadate upon calcium-stimulated ATPase of the rabbit iris-ciliary body

R. R. Socci, Nicholas A Delamere

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In many tissues, the level of cytoplasmic calcium mediates cell function. Since cytoplasmic calcium is often maintained at a low level by active calcium extrusion, we examined whether calcium-stimulated ATPase is present in the rabbit ciliary epithelium. A technique was developed to measure calcium-stimulated ATPase in a partially enriched plasma membrane preparation. The enhancement of Na,K-ATPase activity was used to indicate the enrichment of plasma membrane. Marked stimulation of ATPase activity by calcium was observed over a range of calcium concentrations (10-8 to 10-3 M). The calcium concentration necessary to elicit half-maximal ATPase activity was 10-6 M, which is similar to that reported for other membrane preparations. Calcium-stimulated ATPase activity was significantly inhibited in the presence of low concentrations of sodium orthovanadate. The inhibitory influence of vanadate was examined over a range of vanadate concentrations (10-8 to 10-3 M). The vanadate concentration needed to produce half-maximal inhibition of calcium-stimulated ATPase was 2 x 10-6 M. These studies show that calcium-stimulated ATPase inhibition can occur, in vitro, at very low levels of vanadate; it is possible that this might contribute to the chain of events which results in the lowering of aqueous humor secretion reported in vanadate-treated rabbits.

Original languageEnglish (US)
Pages (from-to)1866-1870
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume29
Issue number12
StatePublished - 1988
Externally publishedYes

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Ciliary Body
Vanadates
Calcium-Transporting ATPases
Iris
Rabbits
Calcium
Adenosine Triphosphatases
Cell Membrane
Aqueous Humor
Epithelium
Sodium
Membranes

ASJC Scopus subject areas

  • Ophthalmology

Cite this

The effect of vanadate upon calcium-stimulated ATPase of the rabbit iris-ciliary body. / Socci, R. R.; Delamere, Nicholas A.

In: Investigative Ophthalmology and Visual Science, Vol. 29, No. 12, 1988, p. 1866-1870.

Research output: Contribution to journalArticle

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AB - In many tissues, the level of cytoplasmic calcium mediates cell function. Since cytoplasmic calcium is often maintained at a low level by active calcium extrusion, we examined whether calcium-stimulated ATPase is present in the rabbit ciliary epithelium. A technique was developed to measure calcium-stimulated ATPase in a partially enriched plasma membrane preparation. The enhancement of Na,K-ATPase activity was used to indicate the enrichment of plasma membrane. Marked stimulation of ATPase activity by calcium was observed over a range of calcium concentrations (10-8 to 10-3 M). The calcium concentration necessary to elicit half-maximal ATPase activity was 10-6 M, which is similar to that reported for other membrane preparations. Calcium-stimulated ATPase activity was significantly inhibited in the presence of low concentrations of sodium orthovanadate. The inhibitory influence of vanadate was examined over a range of vanadate concentrations (10-8 to 10-3 M). The vanadate concentration needed to produce half-maximal inhibition of calcium-stimulated ATPase was 2 x 10-6 M. These studies show that calcium-stimulated ATPase inhibition can occur, in vitro, at very low levels of vanadate; it is possible that this might contribute to the chain of events which results in the lowering of aqueous humor secretion reported in vanadate-treated rabbits.

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