The effect of zinc and other metals on complement hemolysis of sheep red blood cells in vitro

David W Montgomery, L. K. Don, C. F. Zukoski, M. Chvapil

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Abstract

Several transition series and IIB group metals were evaluated for effects on complement hemolysis of sheep red blood cells in vitro. Zinc and copper (10-4 M) strongly inhibited complement activity by 83% and 66%, respectively. However, while manganese inhibited slightly (17%), neither cobalt nor cadmium had effect in this concentration range. Further results showed that zinc pretreatment did not inactivate individual reaction components, and that zinc did not affect the membrane lysis step. Rather, zinc exerted its effect only when added concomitantly with complement, suggesting that it interferes with the binding and/or activation steps necessary to form the complete complement complex.

Original languageEnglish (US)
Pages (from-to)263-267
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume145
Issue number1
StatePublished - 1974

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Hemolysis
Zinc
Sheep
Blood
Erythrocytes
Metals
Cells
Manganese
Cobalt
Cadmium
Copper
Chemical activation
Membranes
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The effect of zinc and other metals on complement hemolysis of sheep red blood cells in vitro. / Montgomery, David W; Don, L. K.; Zukoski, C. F.; Chvapil, M.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 145, No. 1, 1974, p. 263-267.

Research output: Contribution to journalArticle

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