The effects of bFGF, IGF-I, and TGF-β on RMo skeletal muscle cell proliferation and differentiation

Sally E. Johnson, Ronald E. Allen

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

A new skeletal muscle cell line, rat myoblast ω or RMo, has been characterized with regard to the effects of three growth factors: basic fibroblast growth factor (bFGF), insulin-like growth factor I (IGF-I), and transforming growth factor β (TGF-β). Results indicate a differential response of these factors on both cell proliferation and differentiation. Exposure to bFGF and IGF-I stimulate proliferation, while TGF-β has no effect on cell number. RMo cell differentiation, as indicated by skeletal myosin synthesis, is enhanced by IGF-I, whereas both bFGF and TGF-β suppress differentiation. These responses are in agreement with the effects of bFGF, IGF-I, and TGF-β on myogenic cells cultured from fetal and postnatal muscle, thereby suggesting that RMo cells can serve as a model system for the study of growth factor effects on skeletal muscle cells.

Original languageEnglish (US)
Pages (from-to)250-254
Number of pages5
JournalExperimental Cell Research
Volume187
Issue number2
DOIs
StatePublished - Apr 1990

ASJC Scopus subject areas

  • Cell Biology

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