The effects of hyperoxic and hypercarbic gases on tumour blood flow

T. J. Dunn, R. D. Braun, W. E. Rhemus, G. L. Rosner, Timothy W Secomb, G. M. Tozer, D. J. Chaplin, M. W. Dewhirst

Research output: Contribution to journalArticle

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Abstract

Carbogen (95% O2 and 5% CO2) has been used in preference to 100% oxygen (O2) as a radiosensitizer, because it is believed that CO2 blocks O2-induced vasoconstriction. However, recent work suggests that both normal and tumour arterioles of dorsal flap window chambers exhibit the opposite: no vasoconstriction vs constriction for O2 vs carbogen breathing respectively. We hypothesized that CO2 content might cause vasoconstriction and investigated the effects of three O2-CO2 breathing mixtures on tumour arteriolar diameter (TAD) and blood flow (TBF). Fischer 344 rats with R3230Ac tumours transplanted into window chambers breathed either 1%, 5%, or 10% CO2 + O2. Intravital microscopy and laser Doppler flowmetry were used to measure TAD and TBF respectively. Animals breathing 1% CO2 had increased mean arterial pressure (MAP), no change in heart rate (HR), transient reduction in TAD and no change in TBF. Rats breathing 5% CO2 (carbogen) had transiently increased MAP, decreased HR, reduced TAD and a sustained 25% TBF decrease. Animals exposed to 10% CO2 experienced a transient decrease in MAP, no HR change, reduced TAD and a 30-40% transient TBF decrease. The effects on MAP, HR, TAD and TBF were not CO2 dose-dependent, suggesting that complex physiologic mechanisms are involved. Nevertheless, when ≥ 5% CO2 was breathed, there was clear vasoconstriction and TBF reduction in this model. This suggests that the effects of hypercarbic gases on TBF are site-dependent and that use of carbogen as a radiosensitizer may be counterproductive in certain situations.

Original languageEnglish (US)
Pages (from-to)117-126
Number of pages10
JournalBritish Journal of Cancer
Volume80
Issue number1-2
DOIs
StatePublished - 1999

Fingerprint

Gases
Vasoconstriction
Neoplasms
Arterial Pressure
Respiration
Heart Rate
Laser-Doppler Flowmetry
Heart Neoplasms
Inbred F344 Rats
Arterioles
Constriction
Oxygen
carbogen

Keywords

  • Arteriolar diameter
  • Blood flow
  • Carbogen
  • Carbon dioxide
  • Tumour

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Dunn, T. J., Braun, R. D., Rhemus, W. E., Rosner, G. L., Secomb, T. W., Tozer, G. M., ... Dewhirst, M. W. (1999). The effects of hyperoxic and hypercarbic gases on tumour blood flow. British Journal of Cancer, 80(1-2), 117-126. https://doi.org/10.1038/sj.bjc.6690330

The effects of hyperoxic and hypercarbic gases on tumour blood flow. / Dunn, T. J.; Braun, R. D.; Rhemus, W. E.; Rosner, G. L.; Secomb, Timothy W; Tozer, G. M.; Chaplin, D. J.; Dewhirst, M. W.

In: British Journal of Cancer, Vol. 80, No. 1-2, 1999, p. 117-126.

Research output: Contribution to journalArticle

Dunn, TJ, Braun, RD, Rhemus, WE, Rosner, GL, Secomb, TW, Tozer, GM, Chaplin, DJ & Dewhirst, MW 1999, 'The effects of hyperoxic and hypercarbic gases on tumour blood flow', British Journal of Cancer, vol. 80, no. 1-2, pp. 117-126. https://doi.org/10.1038/sj.bjc.6690330
Dunn, T. J. ; Braun, R. D. ; Rhemus, W. E. ; Rosner, G. L. ; Secomb, Timothy W ; Tozer, G. M. ; Chaplin, D. J. ; Dewhirst, M. W. / The effects of hyperoxic and hypercarbic gases on tumour blood flow. In: British Journal of Cancer. 1999 ; Vol. 80, No. 1-2. pp. 117-126.
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AU - Secomb, Timothy W

AU - Tozer, G. M.

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