Abstract
To investigate the effects of metabolic acidosis on jejunal phosphate and glucose absorption, in vivo and in vitro transport studies were performed on weanling rats fed 1.5% NH4CI for three days and on group pair-fed controls. Both in vivo and in vitro, acidosis significantly depressed phosphate transport without effecting glucose transport. In vitro, the decrease of phosphate transport was due to a depression of sodium-phosphate cotransport, but not of sodium independent phosphate transport. This corresponded to a significant increase of the Km of sodium-phosphate cotransport with no change of the Vmax. Treatment of the acidotic animals with intraperitoneal 1,25 dihydroxycholecalciferol did not restore phosphate transport to control levels. These studies indicate that in weanling rats, metabolic acidosis selectively suppresses jejunal phosphate transport independent of circulating levels of 1,25 dihydroxycholecalciferol.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 763-767 |
| Number of pages | 5 |
| Journal | Pediatric Research |
| Volume | 20 |
| Issue number | 8 |
| State | Published - 1986 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health
Cite this
The effects of metabolic acidosis on jejunal phosphate and glucose transport in weanling rats. / Borowitz, Stephen M.; Said, Hamid M.; Ghishan, Fayez K.
In: Pediatric Research, Vol. 20, No. 8, 1986, p. 763-767.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effects of metabolic acidosis on jejunal phosphate and glucose transport in weanling rats
AU - Borowitz, Stephen M.
AU - Said, Hamid M.
AU - Ghishan, Fayez K
PY - 1986
Y1 - 1986
N2 - To investigate the effects of metabolic acidosis on jejunal phosphate and glucose absorption, in vivo and in vitro transport studies were performed on weanling rats fed 1.5% NH4CI for three days and on group pair-fed controls. Both in vivo and in vitro, acidosis significantly depressed phosphate transport without effecting glucose transport. In vitro, the decrease of phosphate transport was due to a depression of sodium-phosphate cotransport, but not of sodium independent phosphate transport. This corresponded to a significant increase of the Km of sodium-phosphate cotransport with no change of the Vmax. Treatment of the acidotic animals with intraperitoneal 1,25 dihydroxycholecalciferol did not restore phosphate transport to control levels. These studies indicate that in weanling rats, metabolic acidosis selectively suppresses jejunal phosphate transport independent of circulating levels of 1,25 dihydroxycholecalciferol.
AB - To investigate the effects of metabolic acidosis on jejunal phosphate and glucose absorption, in vivo and in vitro transport studies were performed on weanling rats fed 1.5% NH4CI for three days and on group pair-fed controls. Both in vivo and in vitro, acidosis significantly depressed phosphate transport without effecting glucose transport. In vitro, the decrease of phosphate transport was due to a depression of sodium-phosphate cotransport, but not of sodium independent phosphate transport. This corresponded to a significant increase of the Km of sodium-phosphate cotransport with no change of the Vmax. Treatment of the acidotic animals with intraperitoneal 1,25 dihydroxycholecalciferol did not restore phosphate transport to control levels. These studies indicate that in weanling rats, metabolic acidosis selectively suppresses jejunal phosphate transport independent of circulating levels of 1,25 dihydroxycholecalciferol.
UR - http://www.scopus.com/inward/record.url?scp=0022460798&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022460798&partnerID=8YFLogxK
M3 - Article
C2 - 3755523
AN - SCOPUS:0022460798
VL - 20
SP - 763
EP - 767
JO - Pediatric Research
JF - Pediatric Research
SN - 0031-3998
IS - 8
ER -