The fibronectin adhesin of Candida albicans

Stephen A Klotz, R. C. Hein, R. L. Smith, J. B. Rouse

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Candida albicans possesses on its cell surface an adhesin which binds the whole viable fungus to subendothelial extracellular matrix and matrix proteins. The adhesin is composed of 75 to 80% carbohydrate and approximately 20 to 25% protein by weight. High-performance liquid chromatography of material eluted from a fibronectin-agarose affinity column demonstrates the presence of three peaks, all of which on sodium dodecyl sulfate- polyacrylamide gel electrophoresis show the presence of one protein of approximately 60 kDa. Molecular weight sizing column chromatography, however, demonstrates that the adhesin elutes with an apparent molecular mass of 42 kDa. The N terminus of the 60-kDa glycoprotein is blocked to Edman degradation. The fibronectin adhesin of C. albicans is a glycoprotein that may be present and functional as an aggregate or multimer of a 60-kDa protein.

Original languageEnglish (US)
Pages (from-to)4679-4681
Number of pages3
JournalInfection and Immunity
Volume62
Issue number10
StatePublished - 1994
Externally publishedYes

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Fibronectins
Glycoproteins
Proteins
Candida albicans
Sodium Dodecyl Sulfate
Sepharose
Extracellular Matrix
Chromatography
Polyacrylamide Gel Electrophoresis
Fungi
Molecular Weight
High Pressure Liquid Chromatography
Carbohydrates
Weights and Measures
Candida albicans ALA1 protein

ASJC Scopus subject areas

  • Immunology

Cite this

Klotz, S. A., Hein, R. C., Smith, R. L., & Rouse, J. B. (1994). The fibronectin adhesin of Candida albicans. Infection and Immunity, 62(10), 4679-4681.

The fibronectin adhesin of Candida albicans. / Klotz, Stephen A; Hein, R. C.; Smith, R. L.; Rouse, J. B.

In: Infection and Immunity, Vol. 62, No. 10, 1994, p. 4679-4681.

Research output: Contribution to journalArticle

Klotz, SA, Hein, RC, Smith, RL & Rouse, JB 1994, 'The fibronectin adhesin of Candida albicans', Infection and Immunity, vol. 62, no. 10, pp. 4679-4681.
Klotz SA, Hein RC, Smith RL, Rouse JB. The fibronectin adhesin of Candida albicans. Infection and Immunity. 1994;62(10):4679-4681.
Klotz, Stephen A ; Hein, R. C. ; Smith, R. L. ; Rouse, J. B. / The fibronectin adhesin of Candida albicans. In: Infection and Immunity. 1994 ; Vol. 62, No. 10. pp. 4679-4681.
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